Extracta Plus DNA

Rapid extraction and purification of high-quality total DNA from cultured cells or tissue

Features & Benefits

  • High yield – Obtain large quantities of high quality DNA, free from PCR inhibitors
  • Sample type versatility – Rapid purification of fresh or frozen tissue, cells, blood or bacteria
  • Lab friendly – Silica based column eliminates toxic organic chemicals such as phenol/chloroform
  • Downstream compatibility – Ideal for sensitive applications such as endpoint PCR, qPCR and next generation sequencing

Extracta Plus DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Extracta Plus DNA kits offer rapid purification of total DNA from a variety of sample sources. Genomic, mitochondrial and pathogenic DNA can be collected from fresh/frozen animal cells, tissue, blood, and bacteria. The Extracta Plus Spin Column workflow enables simultaneous processing of multiple samples into purified DNA suitable for PCR, Southern blotting, RAPD, AFLP and next generation sequencing applications.

Workflow

The efficient, reproducible extraction process allows high yielding extraction of total DNA from various tissues, cells and blood samples.

Extracta Plus DNA complete workflow


Extracta Plus DNA complete workflow

This procedure can be completed in just 15 mins, following sample pre-treatment, resulting in ready-to-use DNA for sensitive downstream applications such as endpoint PCR, qPCR and NGS.


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  • Procedure can be completed in 15 minutes following sample pre-treatment
  • Applicable for sensitive downstream applications such as endpoint PCR, qPCR and NGS


High Quality Extracted DNA

Extracted DNA is free of PCR inhibitors that may impact downstream applications such as PCR, qPCR and next generation sequencing.

Extracted gDNA suitable for qPCR


Extracted gDNA suitable for qPCR

gDNA was extracted from HEK293 cells using the Extracta Plus DNA Kit (blue) and compared to commercially available high quality gDNA (orange). Serial dilutions from 30 ng – 30 pg were prepared and analyzed by qPCR using PerfeCTa SYBR Green FastMix, Low ROX. Linear amplification of diluted samples demonstrates high quality DNA input. The lack of shift in Cq values indicates the absence in interfering inhibitors in the extracted samples.


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  • gDNA extracted from HEK293 cells comparing Extracta Plus DNA Kit (Blue) to commercially available high quality gDNA (orange)

Reproducible preparation of high quality NGS libraries


Reproducible preparation of high quality NGS libraries

gDNA was extracted from mouse whole blood (100 μl) and HEK293 cells (1 x 106 cells) using the Extracta Plus DNA Kit. Illumina NGS libraries were prepared from 100 ng of extracted DNA and an equivalent quantity of commercially available high-quality DNA using the sparQ DNA Frag & Library Prep Kit. TapeStation electrophoresis analysis demonstrates a similar fragmentation pattern and yield for all samples, indicating suitability for NGS library preparation.


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  • TapeStation electrophoresis indicates the high-quality nature of the extracted DNA and suitability for library preparation and NGS sequencing


Extraction of High Molecular Weight DNA

Extracta Plus DNA allows high molecular weight gDNA extraction, enabling long range PCR and sequencing applications.

Extracted gDNA of high molecular weight


Extracted gDNA of high molecular weight

The Extracta Plus DNA Kit was used to extract DNA from a variety of tissues: rat (brain and lung), pig (mammary gland), cow (kidney), mouse (liver, testis and tail snip) and guinea pig (stomach and ovary). Samples were digested with EcoRI and visualized by agarose gel electrophoresis. M: Marker, HindIII digest of lambda DNA.


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  • Extracted DNA from various tissues including rat (brain and lung), pig (mammary gland), cow (kidney), mouse (liver, testis and tail snip) and guinea pig (stomach ovary)
  • Undigested (left) DNA and digested (right) DNA was visualized using agarose gel electrophoresis

High molecular weight gDNA supports long range PCR.


High molecular weight gDNA supports long range PCR

gDNA was extracted from rat kidney or liver samples and 6, 8, 12 or 20 kb targets were amplified using repliQa HiFi ToughMix. Analysis of PCR products by agarose gel electrophoresis demonstrated clean product for each target, comparable to that of commercially available high quality rat gDNA. The yields are similar, indicating high molecular weight and high quality, free of damage, nicks, etc.


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  • High quality yields free of damage, nicks, etc.
  • PCR amplification via repliQa HiFi ToughMix


Performance Data by Sample (Type)

Typical yields from various input samples


High yielding gDNA extraction.

High yielding extraction of total DNA (e.g. genomic, mitochondrial and pathogen) from various tissues as well as cultured cells and blood samples.


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