With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies.
AccuStart II PCR Genotyping Kit
Completely reagent-based system enables reliable PCR genotyping with minimal pipetting skill
Features & Benefits
- Simplified, completely reagent-based system requires minimal pipetting skill
- Premixed electrophretic mobility loading dye reduces chances for post-PCR cross contamination
- Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X)
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance
- Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification
AccuStart II PCR Genotyping Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Description
The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It combines a rapid, 2-component DNA extraction reagent with a user-friendly 2X concentrated PCR SuperMix with loading dye for seamless gel electrophoresis analysis. qPCR-grade genomic DNA template is obtained with minimal extraction volumes (≤ 100uL) and can be carried out in ≤ 30-minutes on a standard PCR thermal cycler.
Performance Data
Accustart II vs KAPA: Mouse tail snips

AccuStart II Results more specific
KAPA PCR mix requires more optimization to reduce non-specific amplification products in multiplex PCR reactions. Lanes: 1 = mouse 1, ZPBP1. 2 = mouse 2, ZPBP1. 3 = mouse 1, ZPBP1/p53. 4 = mouse 2, ZPBP1/p53. 5 = mouse 1, p53. 6 = mouse 2, p53
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What Customers Say
We have tested several different PCR Mastermixes for genotyping in the past four months. Accustart II was the most convincing one concerning quality and price."
Research Scientist
Medical University of Vienna
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AccuStart II PCR Genotyping Kit
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