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AccuStart II PCR Genotyping Kit

4.8
8 reviews
Completely reagent-based system enables reliable PCR genotyping with minimal pipetting skill
Features & Benefits
  • Simplified, completely reagent-based system requires minimal pipetting skill
  • Premixed electrophretic mobility loading dye reduces chances for post-PCR cross contamination
  • Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X)
  • High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance
  • Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification

 

AccuStart II PCR Genotyping Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Part Number
Price
Quantity
 
Product
Kit Size
Order Info
Price
Quantity

AccuStart II PCR Genotyping Kit

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Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  100 x 25 μL rxns
Part Number:  95135-100
$121.00
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Kit Size:  500 x 25 μL rxns
Part Number:  95135-500
$507.00
Add to Cart
To place your order through VWR, click here

Description

The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It combines a rapid, 2-component DNA extraction reagent with a user-friendly 2X concentrated PCR SuperMix with loading dye for seamless gel electrophoresis analysis. qPCR-grade genomic DNA template is obtained with minimal extraction volumes (100uL) and can be carried out in 30-minutes on a standard PCR thermal cycler.

What Customers Say

We have tested several different PCR Mastermixes for genotyping in the past four months. Accustart II was the most convincing one concerning quality and price.”

Medical University of Vienna
Research Scientist
Details

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).
Performance Data

Performance Data

AccuStart II Results more specific
Resource Panel
Customer Testimonials

Customer Testimonials

AccuStart II PCR Genotyping Kit
This kit greatly improved my workflow. Not only did this kit reduce total time from 2 days to just 4 hours, overall ease of use greatly increased. It includes everything I was looking for in one convenient kit - loading dye included in the master mix for quick loading, direct PCR tissue digest, long-term storage stability of DNA extracts, and bright clean bands in a gel as a result.
Lab Manager | Boston University School of Medicine
AccuStart II PCR Genotyping Kit

"With this kit, the genotyping process takes much shorter time and is much easier than before."

Research Scientist | The Ohio State University
AccuStart II PCR Genotyping Kit

"Much quicker process for genotyping, instead of having to go through extra steps for DNA extraction and purification, it's two simple steps to extract DNA and stabilize, then just set up a PCR reaction with the reagents provided, and I get great results"

Research Assistant | UMass Medical School
AccuStart II PCR Genotyping Kit

"This kit has streamlined our genotyping workflow. Easy and fast, we are very pleased with this kit!"

Assistant Professor | Mayo Clinic
AccuStart II PCR Genotyping Kit

"Very intuitive and easy to use. This has greatly sped up our genotyping protocol based on mouse tail snips."

Principal Investigator | Children’s Hospital of Philadelphia
AccuStart II PCR Genotyping Kit

"The AccuStart kit is very easy to use and gives reliable and consistent results. We are happy to switch from our current protocol to AccuStart!"

Assistant Professor | University of Illinois at Urbana-Champaign
AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. | Childrens National Medical Center
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).

Performance Data

AccuStart II Results more specific

Resources

Product Manuals
Manual AccuStart II Mouse Genotyping

CofA (PSFs)
PSF-95135-100-Lot-66283681.pdf
PSF-95135-100-Lot-66280423.pdf
PSF-95135-500-LOT-66277559.pdf
PSF-95135-500-Lot-66272186.pdf
PSF-95135-100-lot-66271800.pdf
PSF-95135-500-LOT-66269191.pdf
PSF-95135-100-lot-66267674.pdf
PSF-95135-500-LOT-66251421.pdf
PSF-95135-100-LOT-66255712.pdf
PSF-95135-100-LOT-66242608.pdf
PSF-95135-500-LOT-66242040.pdf
PSF-95135-500-LOT-66241008.pdf
PSF-95135-100-LOT-66241545.pdf
PSF-95135-100-LOT-66234962.pdf
PSF-95135-100-LOT-66232772.pdf
PSF-95135-500-LOT-66231416.pdf
PSF-95135-100-LOT-66229467.pdf
PSF-95135-500-LOT-66221220.pdf
PSF-95135-100-LOT-66218137.pdf
PSF-95135-500-LOT-66215647.pdf
PSF-95135-500-LOT-66212212.pdf
PSF-95135-100-LOT-66210373.pdf
PSF-95135-500-LOT-66206344.pdf
PSF-95135-500-LOT-66199577.pdf
PSF-95135-500-LOT-66196093.pdf
PSF-95135-100-LOT-66200961.pdf
PSF-95135-100-LOT-66199799.pdf
PSF-95135-100-LOT66161701.pdf
PSF-95135-100-LOT66151673.pdf
PSF-95135-100-LOT66140245.pdf
PSF-95135-100-Lot029828.pdf
PSF-95135-100-Lot027937.pdf
PSf-95135-100-Lot027762.pdf
PSF-95135-100-Lot026681.pdf
PSF-95135-100-Lot025324.pdf
PSF-95135-100-Lot2366161701.pdf
Click here to see all CofA (PSFs)

SDSs
SDS 95135-100 AccuStart II Mouse Genotyping Kit, 100R
SDS 95135-500 AccuStart II Mouse Genotyping Kit, 500R

Publications

Customer Product Reviews
4.8
Based on 8 reviews
5 star
75
75%
4 star
25
25%
3 star
0%
2 star
0%
1 star
0%
1-5 of 8 reviews
  1. Meng W. (Verified)
    Cornell University
    Reviewed on

    The AcuuStart II Mouse Genotyping Kit 100R has worked excellent in our current mouse genotyping protocols. The overall sensitivity was improved immensely. Additionally, the kit decreased the amount of time needed to prepare the DNA for PCR amplification.

  2. Steve C. (Verified)
    Binghamton University
    Reviewed on

    We have previously tried the extraction system, and we are very satisfied with the system. This time, we tested the efficacy of the PCR system. The PCR results were robust, and the procedure was straightforward.

  3. Ben S. (Verified)
    Cornell University
    Reviewed on

    The speed and ease of this for extracting DNA from fish fin clips is amazing! We’re getting clean qPCR (genotyping) results and haven’t had a sample fail yet! Best yet is that it only takes 30min, compared to our previous workflow which took a few hours and had ~80% success rate.

  4. Shuqi W. (Verified)
    LSU Health Shreveport
    Reviewed on
  5. Ping X. (Verified)
    AbCellera
    Reviewed on
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Customer Testimonials

AccuStart II PCR Genotyping Kit
This kit greatly improved my workflow. Not only did this kit reduce total time from 2 days to just 4 hours, overall ease of use greatly increased. It includes everything I was looking for in one convenient kit - loading dye included in the master mix for quick loading, direct PCR tissue digest, long-term storage stability of DNA extracts, and bright clean bands in a gel as a result.
Lab Manager | Boston University School of Medicine
AccuStart II PCR Genotyping Kit

"With this kit, the genotyping process takes much shorter time and is much easier than before."

Research Scientist | The Ohio State University
AccuStart II PCR Genotyping Kit

"Much quicker process for genotyping, instead of having to go through extra steps for DNA extraction and purification, it's two simple steps to extract DNA and stabilize, then just set up a PCR reaction with the reagents provided, and I get great results"

Research Assistant | UMass Medical School
AccuStart II PCR Genotyping Kit

"This kit has streamlined our genotyping workflow. Easy and fast, we are very pleased with this kit!"

Assistant Professor | Mayo Clinic
AccuStart II PCR Genotyping Kit

"Very intuitive and easy to use. This has greatly sped up our genotyping protocol based on mouse tail snips."

Principal Investigator | Children’s Hospital of Philadelphia
AccuStart II PCR Genotyping Kit

"The AccuStart kit is very easy to use and gives reliable and consistent results. We are happy to switch from our current protocol to AccuStart!"

Assistant Professor | University of Illinois at Urbana-Champaign
AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. | Childrens National Medical Center
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

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