sparQ Lysis Kit
- Rapid workflow: Extract high-quality gDNA in 50 minutes, saving time and increasing lab efficiency
- High molecular weight gDNA: Yields intact genomic DNA (>50 kb) ideal for long-read sequencing
- Versatile sample compatibility: one kit supports a broad range of sample types, including DBS, whole blood, saliva
- Broad application use: delivers DNA ready for NGS (short and long-read), qPCR, dPCR, and other genomic workflows
- Automation-ready: compatible with high-throughput and automated platforms for effortless scalability
sparQ Lysis Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
sparQ Lysis Kit
Description
The sparQ Lysis Kit offers a fast and efficient method for extracting high-molecular-weight genomic DNA (>50 kb) from a variety of sample types, including dried blood spots (DBS), whole blood, and saliva—in just 50 minutes. Designed for both manual and automated platforms, this streamlined workflow delivers consistent, high-yield results and is optimized for high-throughput processing.
As a key component of the sparQ DBS-seq workflow, the sparQ Lysis Kit enables the extraction of high-quality gDNA for next-generation sequencing (NGS), as well as qPCR, dPCR, and other genomic applications – even from limited sample input.
Details
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|
Volume |
|
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Component Description |
95220-024 |
95220-096 |
|
DBSL Buffer |
1 x 1.2 mL |
4 x 1.2 mL |
|
Proteinase K |
1 x 240 μl |
1 x 960 μl |
|
Elution Buffer |
1 x 1.32 mL |
4 x 1.32 mL |
Performance Data
sparQ Lysis Kit workflow
sparQ Lysis Kit workflow.
The efficient protocol enables extraction of high-quality, high-molecular-weight gDNA in just 50 minutes from DBS punches.
The workflow is simple, automation-friendly, and delivers DNA suitable for NGS, qPCR, dPCR and other genomic applications.
gDNA Extraction from Diverse Sample Types
High-quality gDNA extracted from diverse sample types.
Samples tested included three 3.2 mm punches of dried blood spots (DBS) collected from adult, newborn, and cord blood; 200 ul of whole adult blood; 400 ul of stabilized saliva; and 400 ul of stabilized cheek swab. The presence of distinct, high molecular weight bands indicates intact, high-purity DNA suitable for downstream molecular analyses.
Maximized Yield from Minimized Input
gDNA yield correlated with the number of DBS punches.
Three punches yielded an average of 175 ng of gDNA, suitable for PCR-free whole
genome library prep. Additional punches can increase yield for applications that demands higher input.
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