Quantabio’s repliQa HiFi ToughMix selected as the enzyme of choice for NGS library amplification in Wellcome Sanger Benchmarking Study
Researchers from the Wellcome Sanger Institute have benchmarked over 20 high-fidelity enzymes for next-generation sequencing library amplification, shedding light on the best PCR polymerases and products for both short-read and long-read applications.
Of the roughly two dozen enzymes tested, they found that the Quantabio RepliQa HiFi ToughMix, the Watchmaker Genomics Equinox Amplification Master Mix, and the Takara Ex Premier DNA polymerase were the “enzymes of choice” for amplifying short-read Illumina libraries. Meanwhile, Quantabio RepliQa also trumped other candidates for long-read library amplification