repliQa HiFi ToughMix
- Fidelity: >90x wild type Taq
- Extreme Speed: Up to 3x faster PCR results with extension rates as fast as 1sec/ kb*
- Tough Tested: Tolerant to a wide range of PCR inhibitors
- Superior Sensitivity: Higher yields from low inputs for both singleplex and multiplex PCR
- Long Range: Amplify targets up to 40 kb
- Broad GC Compatibility: Reliable amplification across varied GC-content
*for fragments less than 1 kb in size
repliQa HiFi ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
repliQa HiFi ToughMix
Description
repliQa HiFi ToughMix is a 2x ready-to-use master mix for high-fidelity PCR, combining a genetically engineered polymerase with hot-start antibody technology. Its optimized chemistry supports reliable amplification from challenging samples and consistent performance across singleplex and multiplex PCR, making it ideal for cloning, amplicon sequencing, library amplification, and site-directed mutagenesis.
Same Product. Expanded Capabilities.
Demonstrated for robust performance in both singleplex and multiplex PCR, enabling broader application across cloning, NGS, and endpoint workflows.
Accelerating Pathogen Detection Across One Health
Explore real‑world applications of 16S metagenomic sequencing for rapid pathogen detection across human, animal, and environmental health.
“Wellcome Sanger Benchmarking Study Highlights Suitable Enzymes for NGS Library Amplification” showcasing the exceptional performance of repliQa HiFi ToughMix. This article is based on the publication released recently in Microbial Genomics.
Details
2x reaction buffer containing optimized concentrations of MgCl2, dNTP’s and proprietarily formulated HiFi polymerase, hot start antibodies and ToughMix chemistry
Performance Data
Extreme Speed: Up to 3x faster results
Comparison of speed. A 2 kb fragment was amplified in 50 μl reaction volumes according to the recommended protocol. Following a 30 s activation at 98°C; 30 cycles of PCR were performed: 98°C, 10 s; 60°C, 10 s; 68°C, 5–30 s. The thermal cycler had a ramp rate of 5°C/s.
Tough Tested: Tolerant to a wide range of PCR inhibitors
Strong Inhibitor Resistance. A 2 kb λ DNA template was amplified using each manufacturer’s recommended cycling conditions with different amounts io inhibitors. This experiment was run in duplicate.
Superior Yield and Sensitivity
Comparison of yield. A gDNA template was amplified with varying GC-content and length targets using each manufacturer’s recommended cycling conditions. The experiment was run using 8 different targets, in duplicate.
Reliable Read Mapping in 23-Plex Viral Genome Sequencing
PCR products were generated from SARS-CoV-2 using the 23 primer pairs from the Midnight Amplicon Panel, divided into two primer pools. Following sequencing on Oxford Nanopore Technologies® instrument, comparable read counts and mapping rates across mutliplex and singleplex conditions were demonstrated, regardless of extension time.
Long Amplification and Consistent GC Tolerance
A Long Range Capabilities. A range from 0.25 to 40 kb of gDNA templates were amplified with varying GC-content and lengths using recommended cycling
conditions. B/C Wide GC-content tolerance range. gDNA templates were amplified with varying GC-content and lengths using recommended cycling conditions.
Documents & Downloads
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| 1 star | 0% |
repliQa HiFi ToughMix
Customer Testimonials
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We tried this product on six primer pairs with fragments ranging from 1500 to 2500 basepairs. They would either not work at all or would produce multiple bands when using our current high fidelity taq. This product yielded clean single band products and the PCR reaction only took 40 minutes to run 30 cycles.
The master mix performed well for colony PCR & diagnosis up to 4kb tested. It may not work as well for low template construct amplification up to 10kb (need further testing)