qScript One-Step RT-qPCR Kit
- 1-step convenience
- high efficiency and sensitivity
- available with reference dye pre-mixed
qScript One-Step RT-qPCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
qScript One-Step RT-qPCR Kit
qScript One-Step qRT-PCR Kit, Low-ROX
qScript One-Step qRT-PCR Kit, ROX
Description
The qScript One-Step qRT-PCR Kit is a convenient and highly sensitive solution for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan(r) 5'-hydrolysis probes. cDNA Synthesis and PCR amplification are carried out in the same tube without opening between procedures. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity, enabling unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes. The proprietary reaction buffer has been specifically formulated to maximize activities of both reverse transcriptase and Taq DNA polymerase while minimizing the potential for primer-dimer and other non-specific PCR artifacts.
Details
qScript One-Step Reverse Transcriptase (50x)
One-Step Master Mix 2x (optionally containing ROX or Low ROX)
Nuclease-free water
Instrument Compatibility
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™ (all models)
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Agilent AriaMx
- Azure Cielo™
- Analytik Jena qTower
- Analytik Jena qTOWERiris
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Resources
Product Manuals
Application Notes
CofA (PSFs)
SDSs
Publications
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qScript One-Step RT-qPCR Kit
It has worked great with fresh RNA samples from yeast.