AccuStart II PCR SuperMix
- User-friendly 2X concentrated master mix simplifies reaction setup with exceptional room-temperature stability (≥30 days at 22°C) and is impervious to repetitive freeze-thaw (≥ 20X)
- High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable assay sensitivity
- Stringent, ultrapure antibody hotstart ensures sensitive and precise target amplification
AccuStart II PCR SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
AccuStart II PCR SuperMix
Description
AccuStart II PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart II PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart II Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Details
- 2X reaction buffer containing 3 mM MgCl2
- 0.4 mM each dNTP (dATP, dCTP, dGTP, dTTP)
- AccuStart II Taq DNA Polymerase and stabilizers.
Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
Repeated freezing and thawing does not impair product performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Customer Testimonials
"The SuperMix worked extremely well."
"This worked well for our very difficult primers. This is one the best solution."
"I used the AccuStart II PCR SuperMix for Sanger sequencing and received quality reads exceeding 800 bp even using difficult primers."
"After testing AccuStart II PCR SuperMix, we found that this product have a better sensitivity for complicated and inhibitor-contaminated amplification of difficult samples."
Details
- 2X reaction buffer containing 3 mM MgCl2
- 0.4 mM each dNTP (dATP, dCTP, dGTP, dTTP)
- AccuStart II Taq DNA Polymerase and stabilizers.
Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
Repeated freezing and thawing does not impair product performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
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AccuStart II PCR SuperMix
Customer Testimonials
"The SuperMix worked extremely well."
"This worked well for our very difficult primers. This is one the best solution."
"I used the AccuStart II PCR SuperMix for Sanger sequencing and received quality reads exceeding 800 bp even using difficult primers."
"After testing AccuStart II PCR SuperMix, we found that this product have a better sensitivity for complicated and inhibitor-contaminated amplification of difficult samples."
We seem to have higher yield of PCR product comparatively with the product we use currently. More testing to be done but very good product without a doubt.
The sensitivity for bacterial signal is good, but the background signal in this recent batch is much higher than it’s been in the past.
Oh no and sorry to hear this. We appreciate your feedback. Our tech support and applications teams are reaching out to best support you. We look forward to getting this resolved for you.