First-Strand cDNA Synthesis

Quantabio reverse transcription reagents leverage proprietary, performance-engineered qScript^® reverse transcriptase in a variety of stabilized, user-friendly reagent formulations that maximize cDNA yield and provide linear cDNA synthesis across a broad dynamic range of input RNA. qScript cDNA synthesis kits redefine what is possible in speed, convenience, reproducibility, specificity and limit of detection (LOD) sensitivity in qPCR and RT-PCR applications.

First-Strand cDNA Synthesis comparison chart

	NEW next generation RT enzyme
	qScript Ultra SuperMix	qScript Ultra Flex Kit	qScript cDNA SuperMix	qScript XLT cDNA SuperMix	qScript cDNA Synthesis Kit	qScript Flex cDNA Synthesis Kit

Features
Kit Format	One Tube	Five Tubes (water included)	One Tube	One Tube	Two Tubes	Six Tubes (water included)
RT Enzyme	qScript Ultra RT	qScript Ultra RT	MMLV, RNase H+	MMLV, reduced RNase activity	MMLV, RNase H+	MMLV, RNase H+
Concentration	5x	5x	5x	5x	5x	5x
Yield	++++	++++	+++	++++	++	++
Total RNA Input Range	1 pg – 2.5 µg	1 pg – 2.5 µg	10 pg – 1 µg	1 pg – 1 µg	10 pg – 1 µg	10 pg – 4 µg
Amplicon Length	300 bp or less	20 kb or less	1 kb or less	1 kb or less	1 kb or less	12 kb or less
Optimal Reaction Time	10 min	10 min	40 min	30 – 70 min	40 min	60 – 90 min
Priming Method
Oligo(dt)	x	x	x	x	x	x
Random	x	x	x	x	x	x
Gene Specific		x				x
Applications
High Yield	x	x		x
Available Formats
Master Mix	x		x	x
Packaging (Rxns/Units)	25 Rxns 100 Rxns 500 Rxns	25 Rxns 100 Rxns	25 Rxns 100 Rxns 500 Rxns	25 Rxns 100 Rxns 500 Rxns	25 Rxns 100 Rxns 500 Rxns	25 Rxns 100 Rxns