Next Generation Sequencing (NGS)
Precise quantification of DNA library molecules is the most critical step for attaining high-quality read data with next-generation sequencing (NGS) technologies. PerfeCTa NGS Library Quantification Kit uses real-time PCR to quantify tagged library molecules for either emulsion PCR (Life Technologies - Ion Torrent) or bridge PCR (Illumina).
Quantabio NGS Quantification kits address an overlooked flaw in other supplier kits which rely on overly concentrated DNA standards that generate Cq values outside the linear dynamic range of many qPCR instruments. This error then results in inflated PCR efficiencies and inaccurate quantification of the DNA library and suboptimal flow cell utilization. Quantabio DNA standards are prediluted within a carefully selected range to avoid these artifacts and produce superior standard curves with exceptionally high linear regression correlation coefficients.