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qScript XLT cDNA SuperMix

Premium Performance with SuperMix Benefits
Features & Benefits
  • Stabilized 5X concentrated master mix simplifies reaction assembly and improves accuracy by minimizing pipetting steps
  • Optimized, double pre-primed SuperMix provides equal representation of 5′ and 3′ RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR
  • Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure
  • Perform more qPCR assays from limiting amounts of RNA with 2-3 fold improved cDNA yields over older enzyme technologies
  • Broad linear dynamic detection range spanning up to 108 fold

 

qScript XLT cDNA SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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qScript XLT cDNA SuperMix

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Kit Size:
Part Number:
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Quantity:
Kit Size:  25 x 20 μL rxns (1 x 100 µL)
Part Number:  95161-025
Price:  $211.00
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Kit Size:  100 x 20 μL rxns (1 x 400 µL)
Part Number:  95161-100
Price:  $625.00
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Kit Size:  500 x 20 μL rxns (2 x 1000 µL)
Part Number:  95161-500
Price:  $2,437.00
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Description

qScript XLT cDNA SuperMix is a next-generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy-to-use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase mutant with reduced RNase H activity and improved yield and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than our previous qScript cDNA SuperMix cDNA synthesis kits, which utilize an engineered RNase H(+) reverse transcriptase mutant.

Details

Details

Contents

Single-tube, 5X concentrated reagent containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
  • Recombinant ribonuclease inhibitor protein (RIP)
  • qScript XLT reverse transcriptase
  • Titrated concentrations of random hexamer and oligo(dT) primer
  • Proprietary enzyme stabilizers and performance-enhancing additives
Customer Testimonials

Customer Testimonials

qScript XLT cDNA SuperMix
"I find the convenience of qScript XLT cDNA SuperMix to be a great improvement when time or ease of use are the most important factors in your RT"
Scientist | Genentech
qScript XLT cDNA SuperMix

"qScript XLT gave us a very clean cDNA that upon qPCR allowed a detection of the gene expression 3-8 cycles earlier than in our current RT."

Ernesto D. | UCSF
qScript XLT cDNA SuperMix

"The cDNA was easy to make and prep was of good quality. I have used 1 ug of total RNA as starting material."

Researcher | University of New Mexico

Details

Contents

Single-tube, 5X concentrated reagent containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
  • Recombinant ribonuclease inhibitor protein (RIP)
  • qScript XLT reverse transcriptase
  • Titrated concentrations of random hexamer and oligo(dT) primer
  • Proprietary enzyme stabilizers and performance-enhancing additives

Performance Data

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Application Notes

CofA (PSFs)

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Publications

Customer Testimonials

qScript XLT cDNA SuperMix
"I find the convenience of qScript XLT cDNA SuperMix to be a great improvement when time or ease of use are the most important factors in your RT"
Scientist | Genentech
qScript XLT cDNA SuperMix

"qScript XLT gave us a very clean cDNA that upon qPCR allowed a detection of the gene expression 3-8 cycles earlier than in our current RT."

Ernesto D. | UCSF
qScript XLT cDNA SuperMix

"The cDNA was easy to make and prep was of good quality. I have used 1 ug of total RNA as starting material."

Researcher | University of New Mexico

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