PerfeCTa FastMix II

Convenient, 1-tube reagent solution supports robust and reliable probe-based DNA detection with fast or standard thermal cycling conditions

Features & Benefits

  • Superior assay sensitivity and target precision with ultrapure AccuStart™ II antibody hotstart technology.
  • Easy-to-use 2X concentrated master mixes with AccuVue™ plate loading dye and optimized passive reference dye for simplified reaction setup.
  • Flexible – optimized for both fast and standard thermal cycling conditions.
  • Supports efficient vortex mixing with proprietary anti-foaming technology.
  • Consistent and reliable performance with exceptional reagent stability – uninhibited after 20X freeze/thaw or 30 days at 22°C.


PerfeCTa FastMix II is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.


PerfeCTa FastMix II is an advanced 2X concentrated qPCR SuperMix reagent for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan hydrolysis probes. The kit is provided as a 2X concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. Inert AccuVue plate loading dye helps to minimize pipette error and provides visual confirmation of thorough mixing. A key component of the kit is an ultra pure, processive thermostable DNA polymerase that is free of detectable E. coli DNA. PerfeCTa FastMix II is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples. Formulated for maximum assay precision with an ultra pure, high avidity monoclonal antibody for stringent suppression for reaction assembly at ambient room temperature and complete activity release and minimal activation time (1-3 minutes).


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