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AccuStart II PCR Genotyping Kit

Completely reagent-based system enables reliable PCR genotyping with minimal pipetting skill
Features & Benefits
  • Simplified, completely reagent-based system requires minimal pipetting skill
  • Premixed electrophretic mobility loading dye reduces chances for post-PCR cross contamination
  • Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X)
  • High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance
  • Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification

 

AccuStart II PCR Genotyping Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

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Product
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AccuStart II PCR Genotyping Kit

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Kit Size:  100 x 25 μL rxns
Part Number:  95135-100
Price:  $120.00
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Kit Size:  500 x 25 μL rxns
Part Number:  95135-500
Price:  $496.00
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Description

The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It combines a rapid, 2-component DNA extraction reagent with a user-friendly 2X concentrated PCR SuperMix with loading dye for seamless gel electrophoresis analysis. qPCR-grade genomic DNA template is obtained with minimal extraction volumes (100uL) and can be carried out in 30-minutes on a standard PCR thermal cycler.

What Customers Say

We have tested several different PCR Mastermixes for genotyping in the past four months. Accustart II was the most convincing one concerning quality and price.”

Medical University of Vienna
Research Scientist
Details

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).
Customer Testimonials

Customer Testimonials

AccuStart II PCR Genotyping Kit

"With this kit, the genotyping process takes much shorter time and is much easier than before."

Research Scientist | The Ohio State University
AccuStart II PCR Genotyping Kit

"Much quicker process for genotyping, instead of having to go through extra steps for DNA extraction and purification, it's two simple steps to extract DNA and stabilize, then just set up a PCR reaction with the reagents provided, and I get great results"

Research Assistant | UMass Medical School
AccuStart II PCR Genotyping Kit

"This kit has streamlined our genotyping workflow. Easy and fast, we are very pleased with this kit!"

Assistant Professor | Mayo Clinic
AccuStart II PCR Genotyping Kit

"Very intuitive and easy to use. This has greatly sped up our genotyping protocol based on mouse tail snips."

Principal Investigator | Children’s Hospital of Philadelphia
AccuStart II PCR Genotyping Kit

"The AccuStart kit is very easy to use and gives reliable and consistent results. We are happy to switch from our current protocol to AccuStart!"

Assistant Professor | University of Illinois at Urbana-Champaign
AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. | Childrens National Medical Center
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

Details

Contents
  • Extracta® DNA Prep for PCR (95091-02)
    • Extraction Reagent
    • Stabilization Buffer
  • AccuStart II GelTrack PCR SuperMix (95136-500)
    • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).

Performance Data

Resources

Customer Testimonials

AccuStart II PCR Genotyping Kit

"With this kit, the genotyping process takes much shorter time and is much easier than before."

Research Scientist | The Ohio State University
AccuStart II PCR Genotyping Kit

"Much quicker process for genotyping, instead of having to go through extra steps for DNA extraction and purification, it's two simple steps to extract DNA and stabilize, then just set up a PCR reaction with the reagents provided, and I get great results"

Research Assistant | UMass Medical School
AccuStart II PCR Genotyping Kit

"This kit has streamlined our genotyping workflow. Easy and fast, we are very pleased with this kit!"

Assistant Professor | Mayo Clinic
AccuStart II PCR Genotyping Kit

"Very intuitive and easy to use. This has greatly sped up our genotyping protocol based on mouse tail snips."

Principal Investigator | Children’s Hospital of Philadelphia
AccuStart II PCR Genotyping Kit

"The AccuStart kit is very easy to use and gives reliable and consistent results. We are happy to switch from our current protocol to AccuStart!"

Assistant Professor | University of Illinois at Urbana-Champaign
AccuStart II Genotyping Kit

"The quality of this product is amazing, especially for the enzyme mixture. It can successfully amplify most of the genotyping targets in a very short time frame."

Yinghua L. | Childrens National Medical Center
AccuStart II Genotyping

"With AccuStart II kit we were able to decrease costs, increase yield of our PCR sensitivity while decreasing and optimizing time. It made genotyping much easier, faster, and cheaper for our multiple transgenic colonies."

Laboratory Manager | H. Lee Moffitt Cancer Center

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