AccuStart II PCR Genotyping Kit

Features & Benefits

  • Simplified, completely reagent-based system requires minimal pipetting skill.
  • Premixed electrophretic mobility loading dye reduces chances for post-PCR cross contamination.
  • Stabilized 2X PCR SuperMix enables convenient room-temperature setup and is unaffected by repetitive freeze-thaw (>20X).
  • High-yielding, ultrapure modified Taq DNA polymerase delivers robust, reliable duplex assay performance.
  • Stringent, ultrapure antibody hotstart ensures sensitive and specific target amplification.

 

AccuStart II PCR Genotyping Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

The AccuStart II Genotyping Kit is a complete reagent kit designed to support conventional, end-point PCR-based screening of transgenic animal models commonly used in life science research and is validated for use with mouse, fish, or insect tissue specimens. It combines a rapid, 2-component DNA extraction reagent with a user-friendly 2X concentrated PCR SuperMix with loading dye for seamless gel electrophoresis analysis. qPCR-grade genomic DNA template is obtained with minimal extraction volumes (≤ 100uL) and can be carried out in ≤ 30-minutes on a standard PCR thermal cycler.

Performance Data

Accustart II vs KAPA: Mouse tail snips


AccuStart II Results more specific

KAPA PCR mix requires more optimization to reduce non-specific amplification products in multiplex PCR reactions. Lanes: 1 = mouse 1, ZPBP1. 2 = mouse 2, ZPBP1. 3 = mouse 1, ZPBP1/p53. 4 = mouse 2, ZPBP1/p53. 5 = mouse 1, p53. 6 = mouse 2, p53


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Details

  • Contents
    • Extracta® DNA Prep for PCR (95091-02)
      • Extraction Reagent
      • Stabilization Buffer
    • AccuStart II GelTrack PCR SuperMix (95136-500)
      • 2X concentrated SuperMix containing optimized concentrations of molecular-grade MgCl2, dNTP blend, AccuStart II Taq DNA Polymerase, reaction buffer, stabilizers and electrophoretic mobility dyes (4kb & 50bp).
  • Storage & Handling

    Remove AccuStart II GelTrack PCR SuperMix from the kit box and store separately at or below -20°C. Extracta reagents can be stored at room temperature

    AccuStart II GelTrack PCR SuperMix is stable for 1 year when stored in a constant temperature freezer at or below -20°C. For convenience, it may be stored unfrozen at 4°C for up to 6 months. Repeated freezing and thawing does not impair reagent performance. Thaw completely, pulse vortex to mix and briefly spin down to collect tube contents before opening.

Ordering Information

    Product
    Kit Size
    Order Info
  • AccuStart II PCR Genotyping Kit, 500R
    500 x 25 μL rxns