RT Comparison Chart

  First-Strand cDNA Synthesis Conventional RT-PCR Quantitative RT-qPCR
  qScript cDNA SuperMix qScript XLT cDNA SuperMix qScript cDNA Synthesis Kit qScript Flex cDNA Synthesis Kit qScript XLT 1-Step RT-PCR Kit Qscript lyo 1-step qScript XLT 1-Step RT-qPCR ToughMix qScript One-Step SYBR Green RT-qPCR qScript One-Step RT-qPCR Kit UltraPlex 1-Step ToughMix
Features
Kit Format One Tube One Tube Two Tubes Five Tubes Three Tubes One Tube  One Tube  Two Tubes Two Tubes One Tube
RT Enzyme  MMLV, RNase H+ MMLV, reduced RNase activity MMLV, RNase H+ MMLV, RNase H+ MMLV, RNase H(-) MMLV, reduced RNase activity MMLV, reduced RNase activity MMLV, RNase H+ MMLV, RNase H+ MMLV, reduced RNase activity
Concentration  5x 5x 5x 5x 2x N/A 2x 2x 2x 4x
Yield +++ ++++ ++ ++  +++ ++++ ++++ +++ +++ ++++
Total RNA Input Range  10pg  – 1ug  1pg  – 2ug  10pg  – 1ug  10pg  – 4ug  1pg - 1ug 0.5pg  – 500 pg 1pg  – 100ng  1pg – 100ng 1pg - 1ug 1pg – 100ng
Amplicon Length 1 kb or less 1 kb or less 1 kb or less 12 kb or less  Up to 2kb 70 – 300bp 70 – 300bp 70 – 200bp 70 – 300bp 70 – 300bp
Optimal Reaction Time 40 min 30 – 70 min 40 min 60 – 90 min N/A N/A N/A N/A N/A N/A
Priming Method
Oligo(dt)            
Random            
Gene Specific                  
Applications
Inhibitor Tolerant               
High Yield             
Multiplex PCR               
Available Formats
Master Mix           
Lyophilized                  
Packaging (Rxns/Units) 25 Rxns
100 Rxns
500 Rxns
25 Rxns
100 Rxns
500 Rxns
25 Rxns
100 Rxns
500 Rxns
25 Rxns
100 Rxns
20 Rxns
200 Rxns
8 Rxns
24 Rxns
100 Rxns
500 Rxns
2000 Rxns
100 Rxns
400 Rxns
50 Rxns
200 Rxns
100 Rxns
500 Rxns
1000 Rxns

RT Comparison Chart

  First-Strand cDNA Synthesis Conventional RT-PCR
  qScript cDNA SuperMix qScript XLT cDNA SuperMix qScript cDNA Synthesis Kit qScript Flex cDNA Synthesis Kit qScript XLT 1-Step RT-PCR Kit
Features
Kit Format One Tube One Tube Two Tubes Five Tubes Three Tubes
RT Enzyme  MMLV, RNase H+ MMLV, reduced RNase activity MMLV, RNase H+ MMLV, RNase H+ MMLV, RNase H(-)
Concentration  5x 5x 5x 5x 2x
Yield +++ ++++ ++ ++ +++
Total RNA Input Range  10pg  – 1ug  1pg  – 2ug  10pg  – 1ug  10pg  – 4ug  1pg - 1ug
Amplicon Length 1 kb or less 1 kb or less 1 kb or less 12 kb or less  Up to 2kb
Optimal Reaction Time 40 min 30 – 70 min 40 min 60 – 90 min N/A
Priming Method
Oligo(dt)  
Random  
Gene Specific        
Applications
Inhibitor Tolerant         
High Yield       
Multiplex PCR           
Available Formats
Master Mix       
Packaging (Rxns/Units) 25 Rxns
100 Rxns
500 Rxns
25 Rxns
100 Rxns
500 Rxns
25 Rxns
100 Rxns
500 Rxns
25 Rxns
100 Rxns
20 Rxns
200 Rxns

RT Comparison Chart

  Quantitative RT-qPCR
  Qscript lyo 1-step qScript XLT 1-Step RT-qPCR ToughMix qScript One-Step SYBR Green RT-qPCR qScript One-Step RT-qPCR Kit UltraPlex 1-Step ToughMix
Features
Kit Format One Tube  One Tube  Two Tubes Two Tubes One Tube
RT Enzyme  MMLV, reduced RNase activity MMLV, reduced RNase activity MMLV, RNase H+ MMLV, RNase H+ MMLV, reduced RNase activity
Concentration  N/A 2x 2x 2x 4x
Yield ++++ ++++ +++ +++ ++++
Total RNA Input Range  0.5pg  – 500 pg 1pg  – 100ng  1pg – 100ng 1pg - 1ug 1pg – 100ng
Amplicon Length 70 – 300bp 70 – 300bp 70 – 200bp 70 – 300bp 70 – 300bp
Optimal Reaction Time N/A N/A N/A N/A N/A
Applications
Inhibitor Tolerant       
High Yield       
Multiplex PCR     
Available Formats
Master Mix     
Lyophilized        
Packaging (Rxns/Units) 8 Rxns
24 Rxns
100 Rxns
500 Rxns
2000 Rxns
100 Rxns
400 Rxns
50 Rxns
200 Rxns
100 Rxns
500 Rxns
1000 Rxns