sparQ DNA Library Prep Kit

Streamlined, versatile single-tube solution for high quality library rep

Features & Benefits

  • Fast, easy single-tube solution completes library prep in 2.5 hours
  • Suitable for a wide range of input amounts from as low as 250 pg
  • Optimized chemistry ensuring superior library prep sensitivity and efficiency
  • Higher library yields compared to other library prep kits
  • High efficiency enables PCR-free workflow from 100 ng input

 

sparQ DNA Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

sparQ DNA Library Prep Kit is optimized for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms. The simplified protocol speeds up library prep to 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 250 pg to 1 μg. DNA polishing reactions are streamlined into a single step to convert fragmented DNA into 5'-phosphorylated and 3'-dA-tailed DNA fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.

 

Streamlined  workflow

sparQ DNA Library Prep workflow


sparQ DNA Library Prep workflow

The streamlined workflow combines DNA polishing and adapter ligation in a single tube for rapid construction of libraries from fragmented DNA. An optional step using the HiFi PCR Master Mix and Primer Mix ensures even library amplification with minimal bias.


<
>

  • Generates sequencing ready library in 2.5 hours with minimal hands-on time
  • Simplified single-tube solution minimizes sample loss


Higher library yields

Higher library yields


Higher library yields

sparQ DNA Library Prep Kit produces high quality libraries from a broad range of DNA inputs with significantly higher yields. Libraries were prepared with Covaris-sheared human genomic DNA (250 bp average size) using kit manufacturers’ instructions. Amplified libraries (6 PCR cycles for 100 ng input DNA and 13 PCR cycles for 1 ng input DNA) were quantified with Qubit fluorometric method.


<
>

  • Unique enzymes engineered for optimal sensitivity and efficiency
  • Over 50% increase in library yields than other library prep kits
  • Compatible with a broad range of DNA inputs from 250 pg to 1 μg


Uniform coverage across challenging regions

Coverage across a wide range of GC-content


Coverage across a wide range of GC-content

PCR-free (dark blue) and amplified libraries (orange) were prepared from 100 ng of microbial genomic DNA of different species using sparQ DNA Library Prep Kit. Libraries were sequenced on Illumina MiSeq and 2 million sequencing reads from each library was analyzed. Coverage uniformity was examined by plotting normalized coverages of the library against GC-content of the targeted genome. GC-content distribution of targeted genomes is indicated by gray line. Both PCR-free and amplified libraries resulted in uniform coverage across a wide range of GC-content..


<
>

  • Even coverage across a broad GC-content
  • Coverage of amplified libraries closely resembles coverage of PCR-free workflow


High quality sequencing metrics

Higher quality sequencing metrics


Higher quality sequencing metrics

sparQ DNA Library Prep Kit generates high quality DNA libraries with minimal duplication rates. Libraries were prepared with 1 ng and 100 ng of microbial genomic DNA and subsequently sequenced on Illumina MiSeq. Each library was down-sampled to 2 million reads (150 bp paired-end reads) and aligned to a reference genome with only unique alignments reported.


<
>

  • High mapping percentage and low duplication artifacts
  • Industry leading sequencing results ensures better return on investments


sparQ Adapter Barcode Sets

sparQ Adapter Barcode Sets A and B are single index barcoded oligonucleotide adapters designed for use with the Quantabio sparQ DNA Library Prep Kits in the construction of libraries for Illumina NGS platforms. Each sparQ Adapter Barcode Set contains 12 single index barcodes for 96 reactions.s.

sparQ Adapter Barcode Set A
Barcode Number      Barcode Sequence
2 CGATGT
4 TGACCA
5 ACAGTG
6 GCCAAT
7 CAGATC
12 CTTGTA
13 AGTCAA
14 AGTTCC
15 ATGTCA
16 CCGTCC
18 GTCCGC
19 GTGAAA
sparQ Adapter Barcode Set B
Barcode Number  Barcode Sequence
1 ATCACG
3 TTAGGC
8 ACTTGA
9 GATCAG
10 TAGCTT
11 GGCTAC
20 GTGGCC
21 GTTTCG
22 CGTACG
23 GAGTGG
25 ACTGAT
27 ATTCCT

Performance Data

sparQ DNA Library Prep Workflow


sparQ DNA Library Prep Workflow

The streamlined workflow can be completed in under 3 hours with minimal hands-on time. A single tube is used for DNA polishing, ligation, and cleanup. A second tube is used for workflows requiring PCR amplification and a final tube receives the sequencing-ready library.


<
>

Coverage Across Wide Range of GC-Content


Uniform coverage across wide range of GC-content

Library amplification with sparQ HiFi PCR Master Mix contained in the sparQ DNA Library Prep Kit resulted in uniform coverage across the wide range of GC-content. Libraries were prepared by using sparQ DNA Library Prep Kit with 100 ng input DNA. Coverage depth against GC-content of libraries amplified by sparQ HiFi PCR Master Mix (orange) were compared to corresponding libraries without amplification (dark blue: PCR-free library). GC content distribution of targeted genomes is indicated by gray line.


<
>

Improve Sequencing Results and Economics


Improve Sequencing Results and Economics

sparQ DNA Library Prep Kit generates high quality DNA libraries with minimal duplication rates. Libraries were prepared with 1 ng and 100 ng of microbial genomic DNA and subsequently sequenced on Illumina MiSeq. Each library was down-sampled to 2 million reads (150 bp paired-end reads) and aligned to a reference genome with only unique alignments reported.


<
>

Coverage Across Challenging Regions


Coverage Across Challenging Regions

Amplified libraries were prepared from 100 ng of microbial genomic DNA and subsequently sequenced on Illumina MiSeq. 2 million reads from each tested library were down-sampled and analyzed. Coverage uniformity for different library preparation kits were compared by plotting normalized coverage for both extreme AT-rich regions (8%-20% GC-content) and GC-rich regions (75%-88% GC-content).


<
>

sparQ DNA Library Prep Kit Yield Analysis


sparQ DNA Library Prep Kit Yield Analysis

sparQ DNA Library Prep Kit produces high quality libraries from a broad range of DNA inputs with significantly higher yields. Libraries were prepared with Covaris-sheared DNA (250 bp average size) using kit manufacturers’ instructions. Amplified libraries (6 amplification cycles for 100 ng input DNA and 13 amplification cycles for 1 ng input DNA) were quantified with Qubit fluorometric quantitation method.


<
>

sparQ NGS Library Prep Solutions

Details

  • Contents

    featured-product-background.jpg

    1800x670 px.
    featured-product-bac...

    ISO_Certificate.gif

    160x225 px.
    ISO_Certificate.gif

    reproducible-chart-2.jpg

    1200x316 px.
    reproducible-chart-2...

    reproducible-chart-1.jpg

    1200x592 px.
    reproducible-chart-1...

    toughmix-chart-1.jpg

    1159x295 px.
    toughmix-chart-1.jpg

    reproducible-chart-3.jpg

    1200x688 px.
    reproducible-chart-3...

    microrna_detection.gif

    500x549 px.
    microrna_detection.g...

    new_mirna_steps.PNG

    1302x812 px.
    new_mirna_steps.PNG

    new_tech_doc_thumbnail.PNG

    650x867 px.
    new_tech_doc_thumbna...

    mei-baker-testimonial.JPG

    1152x190 px.
    mei-baker-testimonia...

    description_of_dilution-of-assembly-not-required_graph.png

    566x110 px.
    description_of_dilut...

    test.jpg

    2244x1063 px.
    test.jpg

    sparQ_Library_Prep_workflow.jpg

    1063x461 px.
    sparQ_Library_Prep_w...

    Library_Yield_Analysis.jpg

    1063x827 px.
    Library_Yield_Analys...

    Table.jpg

    2126x650 px.
    Table.jpg

    Coverage.jpg

    1063x768 px.
    Coverage.jpg

    GC_Distribution.jpg

    2126x1299 px.
    GC_Distribution.jpg

    GC_Distribution_small.jpg

    1181x722 px.
    GC_Distribution_smal...

    LibraryYieldAnalysis_icon.jpg

    135x100 px.
    LibraryYieldAnalysis...

    data_table_icon.jpg

    120x100 px.
    data_table_icon.jpg

    uniform_coverage_icon.jpg

    115x100 px.
    uniform_coverage_ico...

    Coverage_Regions_icon.jpg

    146x100 px.
    Coverage_Regions_ico...

    Workflow_icon.jpg

    120x100 px.
    Workflow_icon.jpg

    LibraryYieldAnalysis_icon_1.jpg

    146x100 px.
    LibraryYieldAnalysis...

    LibraryYieldAnalysis_icon_2.jpg

    146x100 px.
    LibraryYieldAnalysis...

    Workflow_icon_1.jpg

    146x100 px.
    Workflow_icon_1.jpg

    data_table_icon_1.jpg

    146x100 px.
    data_table_icon_1.jp...

    sparq-graph.png

    881x772 px.
    sparq-graph.png

    uniform_coverage_icon_1.jpg

    140x100 px.
    uniform_coverage_ico...

    sparq-graph-500.png

    571x500 px.
    sparq-graph-500.png

    95191_Library_Yield_promo.jpg

    789x594 px.
    95191_Library_Yield_...

    d_0049_ngs.jpg

    969x691 px.
    d_0049_ngs.jpg

    d_0061_ngs.jpg

    965x690 px.
    d_0061_ngs.jpg

    portable.jpg

    300x300 px.
    portable.jpg

    back.jpg

    250x250 px.
    back.jpg

    Fast.jpg

    300x300 px.
    Fast.jpg

    performance.jpg

    300x300 px.
    performance.jpg

    Magnetic.jpg

    300x300 px.
    Magnetic.jpg

    size2.jpg

    319x227 px.
    size2.jpg

    wireless.jpg

    300x300 px.
    wireless.jpg

    front.jpg

    250x250 px.
    front.jpg

    size1.jpg

    303x227 px.
    size1.jpg

    UltraFastDataAcquisition_1.jpg

    576x500 px.
    UltraFastDataAcquisi...

    Q.jpg

    500x500 px.
    Q.jpg

    dimensions.jpg

    534x192 px.
    dimensions.jpg

    dimension2.jpg

    227x191 px.
    dimension2.jpg

    dimension1.jpg

    227x191 px.
    dimension1.jpg

    q_small.jpg

    600x656 px.
    q_small.jpg

    p_0019_Q_Speed_Box_neutral_free.png

    1743x1980 px.
    p_0019_Q_Speed_Box_n...

    Q.png

    51x52 px.
    Q.png

    Q2.png

    379x467 px.
    Q2.png

    more.jpg

    220x80 px.
    more.jpg

    request_your_demo.jpg

    290x80 px.
    request_your_demo.jp...

    open.jpg

    404x267 px.
    open.jpg

    Qbanner.jpg

    150x185 px.
    Qbanner.jpg

    check.png

    285x297 px.
    check.png

    featured-product-Q-bg.jpeg

    1800x670 px.
    featured-product-Q-b...

    featured-product-Q-bg_1.jpeg

    800x298 px.
    featured-product-Q-b...

    featured-product-Q-bg_2.jpeg

    750x279 px.
    featured-product-Q-b...

    TN_microRNA.jpg

    151x202 px.
    TN_microRNA.jpg

    Q_transparent.png

    124x155 px.
    Q_transparent.png

    Quantabio_Logo_96dpi250_transparent.png

    250x52 px.
    Quantabio_Logo_96dpi...

    ScheduleDemo.png

    300x100 px.
    ScheduleDemo.png

    Learnmore.png

    300x100 px.
    Learnmore.png

    ScheduleDemo_1.png

    300x100 px.
    ScheduleDemo_1.png

    Iso13485Cert.jpg

    620x826 px.
    Iso13485Cert.jpg

    Iso13485Cert_1.jpg

    696x1653 px.
    Iso13485Cert_1.jpg

    Iso13485Cert_2.jpg

    697x915 px.
    Iso13485Cert_2.jpg

    QIAGEN Beverly ISO 13485 certificate.pdf

    QIAGEN Beverly ISO 1...

    qopen_left.jpg

    919x579 px.
    qopen_left.jpg

    Qleft.jpg

    429x354 px.
    Qleft.jpg

    Qleft_1.jpg

    335x597 px.
    Qleft_1.jpg

    Q-icon.png

    16x18 px.
    Q-icon.png

    Enhanced_Stability.jpg

    854x454 px.
    Enhanced_Stability.j...

    quantabio.jpg

    137x29 px.
    quantabio.jpg

    Quantabiologo.jpg

    140x29 px.
    Quantabiologo.jpg

    BOSE_sunglasses_1.jpg

    1920x1080 px.
    BOSE_sunglasses_1.jp...

    ADAPT_shirt.jpg

    1709x996 px.
    ADAPT_shirt.jpg

    NGS_workflow.jpg

    1973x439 px.
    NGS_workflow.jpg

    sparQ_Fast_Lib_Quant.jpg

    905x751 px.
    sparQ_Fast_Lib_Quant...

    image_MK-TN-0011_REV_01_Illumina_NGS_Libraries_using_the_Q_V4.jpg

    1500x1919 px.
    image_MK-TN-0011_REV...

    image_MK-TN-0010_REV_01Library_preparation_solutions_for_NGS.jpg

    300x386 px.
    image_MK-TN-0010_REV...

    cocktail.png

    341x545 px.
    cocktail.png

    Cocktails_Beer.jpg

    800x800 px.
    Cocktails_Beer.jpg

    image_MK-TN-0011_REV_01_Illumina_NGS_Libraries_using_the_Q_V4_1.jpg

    300x384 px.
    image_MK-TN-0011_REV...

    Uniformity.jpg

    576x718 px.
    Uniformity.jpg

    Uniformity_150.jpg

    150x187 px.
    Uniformity_150.jpg

    sparQFargwf.jpg

    576x184 px.
    sparQFargwf.jpg

    sparQFargwf150.jpg

    150x48 px.
    sparQFargwf150.jpg

    sparQFragwf.jpg

    576x184 px.
    sparQFragwf.jpg

    sparQFragwf150.jpg

    150x48 px.
    sparQFragwf150.jpg
  • Storage & Handling
    Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
  • Related Resources

Ordering Information

Related Products