Qscript lyo 1-step

Dry. Stable. Easy. Better. 1-step RT-qPCR

Features & Benefits

Lyophilized Single Tube Format 
Easy-to-use, reduces cross-contamination

High Sensitivity & Specificity 
Detect as low as 0.5 pg RNA

Wide Dynamic Range 
0.5 – 500 pg RNA

Superior Multiplexing 
Plex up to 5 targets per reaction

Eliminate Freezer Storage 
Up to 9 months stability room temperature

Qscript lyo 1-step is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Qscript lyo 1-step is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes. The reagent contains a hot-start thermo-stable polymerase, a genetically engineered reverse transcriptase as well as other components to ensure higher performance detection of up to 5 targets with maximum sensitivity and specificity. The enhanced stability of the freeze-dried master mix enables convenient shipping and storage at room temperature. The single tube reaction facilitates easy reaction set up while preventing potential cross-contamination.


Lyophilized single tube format

Lyophilized single tube format


Convenient reaction setup

Just add RNA, primers and probes


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  • Freeze-dried master mix
  • Easy-to-use workflow
  • Ship and store at ambient temperatures


High sensitivity & specificity

High sensitivity & specificity


High sensitivity & specificity

Qscript lyo 1-step demonstrates higher sensitivity and fluorescence detection than a market-leading one-step RT-qPCR product (liquid). The figure shows an LDHA assay with 10-fold serial diluted universal human RNA (50 ng to 0.5 pg) as template. 20 μl of LDHA primer/probe was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.


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  • Detect as low as 0.5 pg RNA)
  • Greater sensitivity vs leading market RT-qPCR (liquid mastermix)



High performance across a range of inputs

High performance across a range of inputs


High performance across a range of inputs

Qscript lyo 1-step was tested with 12 serial dilutions of Human total RNA (4x from 1250 ng to 0.3 pg). 20 μl of LDHA primer/probe was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.


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  • Wide dynamic range from 0.5 pg – 500 pg



Multiplex up to 5 targets per assay

Multiplex up to 5 targets per assay


Multiplex up to 5 targets per assay

Qscript lyo 1-step was tested with a 5-plex assay with 7 serial dilutions of Human total RNA (4x from 50 ng to 30 pg). 20 μl of primers/probes was used to resuspend each lyosphere, followed by addition of 5 μl of RNA target. RT-qPCR was carried out on a QuantStudio 7 instrument with the following conditions: 50°C 15 min, 95°C 3 min, 40 cycles: 95°C 15 sec, 60°C 60 sec.


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  • Expand assay conditions and targets with reliable multiplexing detection

Details

  • Contents
    Qscript lyo 1-step mastermix (lyosphere)
  • Storage & Handling

    Store the Qscript lyo 1-step in a constant temperature freezer at 4°C upon receipt.

    The kit can also be stored at room temperature for up to 9 months.

    For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

  • Instrument Capability
    ROX
    • Applied Biosystems 5700
    • Applied Biosystems 7000
    • Applied Biosystems 7300
    • Applied Biosystems 7700
    • Applied Biosystems 7900
    • Applied Biosystems 7900HT
    • Applied Biosystems 7900 HT Fast
    • Applied Biosystems StepOne™
    • Applied Biosystems StepOnePlus™
    Low ROX
    • Applied Biosystems 7500
    • Applied Biosystems 7500 Fast
    • Stratagene Mx3000P®
    • Stratagene Mx3005P™
    • Stratagene Mx4000™
    • Applied Biosystems ViiA 7
    • Applied Biosystems QuantStudio™
    • Agilent AriaMx
    • Douglas Scientific IntelliQube®
    • QIAGEN Rotor-Gene Q
    No ROX
    • Quantabio Q
    • BioRad CFX
    • Roche LightCycler 480
    • Other
    Bio-Rad iCycler iQ systems
    • BioRad iCycler iQ™
    • BioRad MyiQ™
    • BioRad iQ™5
  • Related Resources
    Product Flyers
    Product Manuals

Ordering Information