Quantitative RT-qPCR

Quantitative RT-qPCR comparison chart

  Quantitative RT-qPCR
  Qscript lyo 1-step qScript XLT 1-Step RT-qPCR ToughMix qScript One-Step SYBR Green RT-qPCR qScript One-Step RT-qPCR Kit UltraPlex 1-Step ToughMix qScript 1-Step Virus ToughMix
Features
Kit Format One Tube One Tube Two Tubes Two Tubes One Tube One Tube
RT Enzyme  MMLV, reduced RNase activity MMLV, reduced RNase activity MMLV, RNase H+ MMLV, RNase H+ MMLV, reduced RNase activity MMLV, reduced RNase activity
Concentration  N/A 2x 2x 2x 4x 2x
Yield ++++ ++++ +++ +++ ++++ ++++
Total RNA Input Range  0.5pg – 500 pg 1pg – 100ng 1pg – 100ng 1pg - 1ug 1pg – 100ng 1pg – 100ng
Amplicon Length 70 – 300bp 70 – 300bp 70 – 200bp 70 – 300bp 70 – 300bp 70 – 300bp
Hot Start N/A N/A N/A N/A Yes Yes
Applications
Inhibitor Tolerant       
High Yield       
Multiplex PCR     
Available Formats
Master Mix    
Lyophilized          
Packaging (Rxns/Units) 8 Rxns
24 Rxns
100 Rxns
500 Rxns
2000 Rxns
100 Rxns
400 Rxns
50 Rxns
200 Rxns
100 Rxns
500 Rxns
1000 Rxns
100 Rxns
500 Rxns
2000 Rxns

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