Quantitative RT-qPCR
Quantitative RT-qPCR comparison chart
| Quantitative RT-qPCR | |||||
|---|---|---|---|---|---|
| Qscript lyo 1-step | qScript XLT 1-Step RT-qPCR ToughMix | qScript One-Step SYBR Green RT-qPCR | qScript One-Step RT-qPCR Kit | UltraPlex 1-Step ToughMix | |
| Features | |||||
| Kit Format | One Tube | One Tube | Two Tubes | Two Tubes | One Tube |
| RT Enzyme | MMLV, reduced RNase activity | MMLV, reduced RNase activity | MMLV, RNase H+ | MMLV, RNase H+ | MMLV, reduced RNase activity |
| Concentration | N/A | 2x | 2x | 2x | 4x |
| Yield | ++++ | ++++ | +++ | +++ | ++++ |
| Total RNA Input Range | 0.5pg – 500 pg | 1pg – 100ng | 1pg – 100ng | 1pg - 1ug | 1pg – 100ng |
| Amplicon Length | 70 – 300bp | 70 – 300bp | 70 – 200bp | 70 – 300bp | 70 – 300bp |
| Optimal Reaction Time | N/A | N/A | N/A | N/A | N/A |
| Applications | |||||
| Inhibitor Tolerant | • | • | |||
| High Yield | • | • | |||
| Multiplex PCR | • | • | • | ||
| Available Formats | |||||
| Master Mix | • | • | • | ||
| Lyophilized | • | ||||
| Packaging (Rxns/Units) | 8 Rxns 24 Rxns |
100 Rxns 500 Rxns 2000 Rxns |
100 Rxns 400 Rxns |
50 Rxns 200 Rxns |
100 Rxns 500 Rxns 1000 Rxns |
Featured Products
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qScript 1-Step Virus ToughMix
qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses. -
qScript XLT 1-Step RT-qPCR ToughMix
A highly sensitive, ready-to-use master mix for quantitative reverse transcription PCR (RT-qPCR) of RNA templates using probe-based detection methods -
qScript One-Step SYBR Green RT-qPCR
The qScript One-Step SYBR Green RT-qPCR Kit is a convenient and highly sensitive solution for quantitative RT-PCR of RNA templates (RT-qPCR) using SYBR Green I dye detection and gene-specific primers -
UltraPlex 1-Step ToughMix
An advanced 4X concentrated master mix that enables highly sensitive 1-step RNA detection (RT-qPCR) using multiplexed probe-based real-time PCR with crude extracts or inhibitory biological starting materials -
qScript One-Step RT-qPCR Kit
Convenient, reliable 1-step RT-qPCR -
Qscript lyo 1-step
is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes
