Quantitative RT-qPCR
Quantitative RT-qPCR comparison chart
Quantitative RT-qPCR | ||||||
---|---|---|---|---|---|---|
Qscript lyo 1-step | qScript XLT 1-Step RT-qPCR ToughMix | qScript One-Step SYBR Green RT-qPCR | qScript One-Step RT-qPCR Kit | UltraPlex 1-Step ToughMix | qScript 1-Step Virus ToughMix | |
Features | ||||||
Kit Format | One Tube | One Tube | Two Tubes | Two Tubes | One Tube | One Tube |
RT Enzyme | MMLV, reduced RNase activity | MMLV, reduced RNase activity | MMLV, RNase H+ | MMLV, RNase H+ | MMLV, reduced RNase activity | MMLV, reduced RNase activity |
Concentration | N/A | 2x | 2x | 2x | 4x | 2x |
Yield | ++++ | ++++ | +++ | +++ | ++++ | ++++ |
Total RNA Input Range | 0.5pg – 500 pg | 1pg – 100ng | 1pg – 100ng | 1pg - 1ug | 1pg – 100ng | 1pg – 100ng |
Amplicon Length | 70 – 300bp | 70 – 300bp | 70 – 200bp | 70 – 300bp | 70 – 300bp | 70 – 300bp |
Hot Start | N/A | N/A | N/A | N/A | Yes | Yes |
Applications | ||||||
Inhibitor Tolerant | • | • | • | |||
High Yield | • | • | • | |||
Multiplex PCR | • | • | • | • | ||
Available Formats | ||||||
Master Mix | • | • | • | • | ||
Lyophilized | • | |||||
Packaging (Rxns/Units) | 8 Rxns 24 Rxns |
100 Rxns 500 Rxns 2000 Rxns |
100 Rxns 400 Rxns |
50 Rxns 200 Rxns |
100 Rxns 500 Rxns 1000 Rxns |
100 Rxns 500 Rxns 2000 Rxns |
Featured Products
-
qScript 1-Step Virus ToughMix
qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses. -
qScript XLT 1-Step RT-qPCR ToughMix
A highly sensitive, ready-to-use master mix for quantitative reverse transcription PCR (RT-qPCR) of RNA templates using probe-based detection methods -
qScript One-Step SYBR Green RT-qPCR
The qScript One-Step SYBR Green RT-qPCR Kit is a convenient and highly sensitive solution for quantitative RT-PCR of RNA templates (RT-qPCR) using SYBR Green I dye detection and gene-specific primers -
UltraPlex 1-Step ToughMix
An advanced 4X concentrated master mix that enables highly sensitive 1-step RNA detection (RT-qPCR) using multiplexed probe-based real-time PCR with crude extracts or inhibitory biological starting materials -
qScript One-Step RT-qPCR Kit
Convenient, reliable 1-step RT-qPCR -
Qscript lyo 1-step
is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes