Quantitative RT-qPCR

Quantitative RT-qPCR comparison chart

  Quantitative RT-qPCR
  Qscript lyo 1-step qScript XLT 1-Step RT-qPCR ToughMix qScript One-Step SYBR Green RT-qPCR qScript One-Step RT-qPCR Kit UltraPlex 1-Step ToughMix
Features
Kit Format One Tube  One Tube  Two Tubes Two Tubes One Tube
RT Enzyme  MMLV, reduced RNase activity MMLV, reduced RNase activity MMLV, RNase H+ MMLV, RNase H+ MMLV, reduced RNase activity
Concentration  N/A 2x 2x 2x 4x
Yield ++++ ++++ +++ +++ ++++
Total RNA Input Range  0.5pg  – 500 pg 1pg  – 100ng  1pg – 100ng 1pg - 1ug 1pg – 100ng
Amplicon Length 70 – 300bp 70 – 300bp 70 – 200bp 70 – 300bp 70 – 300bp
Optimal Reaction Time N/A N/A N/A N/A N/A
Applications
Inhibitor Tolerant       
High Yield       
Multiplex PCR     
Available Formats
Master Mix     
Lyophilized        
Packaging (Rxns/Units) 8 Rxns
24 Rxns
100 Rxns
500 Rxns
2000 Rxns
100 Rxns
400 Rxns
50 Rxns
200 Rxns
100 Rxns
500 Rxns
1000 Rxns

Featured Products

  • Qscript lyo 1-step
    is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes
  • qScript XLT 1-Step RT-qPCR ToughMix
    A highly sensitive, ready-to-use master mix for quantitative reverse transcription PCR (RT-qPCR) of RNA templates using probe-based detection methods
  • qScript One-Step SYBR Green RT-qPCR
    The qScript One-Step SYBR Green RT-qPCR Kit is a convenient and highly sensitive solution for quantitative RT-PCR of RNA templates (RT-qPCR) using SYBR Green I dye detection and gene-specific primers
  • UltraPlex 1-Step ToughMix
    An advanced 4X concentrated master mix that enables highly sensitive 1-step RNA detection (RT-qPCR) using multiplexed probe-based real-time PCR with crude extracts or inhibitory biological starting materials
  • qScript One-Step RT-qPCR Kit
    Convenient, reliable 1-step RT-qPCR