PerfeCTa DNase I
Simple and rapid removal of residual genomic DNA
Features & Benefits
- Permanent DNase I inactivation for confident first-strand cDNA synthesis with qScript® first-strand cDNA synthesis kits
- Single tube
PerfeCTa DNase I is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
It is not always possible to design qPCR primers with intron spanning sequences to prevent the co-amplification of genomic DNA template. PerfeCTa DNase I is an ultra-pure, recombinant bovine DNase I preparation that is permanently inactivated with a proprietary stop buffer and simple heat kill incubation. This reagent provides a simple and rapid solution to eliminate residual genomic DNA from total RNA preparations for expression profiling by reverse transcription quantitative PCR amplification (RT-qPCR) as well as other molecular biology applications. Complete and permanent DNase I inactivation is critical for linear first-strand cDNA synthesis. Residual or renatured DNase will degrade cDNA product and lead to inaccurate target quantification. One unit completely degrades 1µg of dsDNA in 10 minutes at 37°C.
- PerfeCta DNase I (2 U/µL, recombinant DNase I, RNase-free in, 50 mM glycine (pH 7.2), 5 mM calcium acetate, 50%(v/v) glycerol and stabilizers)
- 10X reaction buffer for DNase I
- 10X Stop Buffer
Storage & HandlingStore components in a constant temperature freezer at -25°C to -15°C upon receipt. However, we recommend that the number of freeze-thaw cycles be kept to a minimum. For convenience, the 10X Reaction Buffer and 10X Stop Buffer may be stored at 2-8˚C to avoid thawing the buffers for each use.