PerfeCTa DNase I

Simple and rapid removal of residual genomic DNA

Features & Benefits

  • Permanent DNase I inactivation for confident first-strand cDNA synthesis with qScript® first-strand cDNA synthesis kits
  • Single tube

PerfeCTa DNase I is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

It is not always possible to design qPCR primers with intron spanning sequences to prevent the co-amplification of genomic DNA template. PerfeCTa DNase I is an ultra-pure, recombinant bovine DNase I preparation that is permanently inactivated with a proprietary stop buffer and simple heat kill incubation. This reagent provides a simple and rapid solution to eliminate residual genomic DNA from total RNA preparations for expression profiling by reverse transcription quantitative PCR amplification (RT-qPCR) as well as other molecular biology applications. Complete and permanent DNase I inactivation is critical for linear first-strand cDNA synthesis. Residual or renatured DNase will degrade cDNA product and lead to inaccurate target quantification. One unit completely degrades 1µg of dsDNA in 10 minutes at 37°C.

Details