Reverse Transcription at Elevated Temperature
Thermostability and Activity at High Temperatures.
cDNA was synthesized in reactions using 5 ng total human RNA and a stable LDHA genespecific reverse primer. Each were incubated at the indicated temperatures, followed by reaction termination with products detected by qPCR. The lower Cq values for the engineered qScript Ultra reverse transcriptase indicate more efficient cDNA synthesis at high temperatures up to 65°C, with no loss in performance up to 60°C, critical for progressing through high GC-regions and regions of RNA secondary structure.