Reverse Transcription Reagents

Quantabio reverse transcription reagents combine advanced qScript RT technology in a variety of stabilized, user-friendly formulations that deliver highly reproducible and sensitive RNA detection across a broad range of RNA input.

Quantabio scientists were the first to successfully develop a comprehensive 1-tube RT reagent with sensitivity and linear dynamic range equivalent to traditional component reaction assembly. New qScript XLT reagents continue to set new boundaries for limit of detection (LOD) sensitivity and reproducibility.

Explore the best qScript for your application.

Which qScript is right for you?

 Unmatched Sensitivity & Yield in a one tube kitSimplicity and Exceptional ValueStrong RT performance with two tube flexibilityComplete reaction control including gene specific priming
cDNA Kit qScript XLT SuperMix qScript SuperMix qScript cDNA Synthesis Kit qScript Flex cDNA Kit
Sensitivity & Yield ++++ +++ ++ ++
Configuration Single tube Single tube Two tube Five tube
Primers Oligo (dT) and random Oligo (dT) and random Oligo (dT) and random Oligo (dT) and random or gene-specific
RNA Input Range 1pg - 2ug 10pg - 1ug 10pg - 1ug 10pg - 1ug
Amplicon Length up to 1kb up to 1kb up to 1kb up to 12kb


  • Qscript lyo 1-step
    is a lyophilized single-reaction reagent optimized for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes
  • qScript cDNA SuperMix
    High performance qScript reverse transcriptase pre-blended with ribonuclease inhibitor protein (RIP) and an optimized primer blend to ensure integrity of low abundance RNA and unbiased sequence representation
  • qScript XLT cDNA SuperMix
    Next-generation qScript XLT reverse transcriptase with novel hot start RT technology in an easy-to-use 5X concentrated master mix for superior 2-step RT-qPCR assay performance and reproducibility
  • qScript XLT 1-Step RT-qPCR ToughMix
    A highly sensitive, ready-to-use master mix for quantitative reverse transcription PCR (RT-qPCR) of RNA templates using probe-based detection methods