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Reverse Transcription at Elevated Temperature

Thermostability and Activity at High Temperatures.
cDNA was synthesized in reactions using 5 ng total human RNA and a stable LDHA genespecific reverse primer. Each were incubated at the indicated temperatures, followed by reaction termination with products detected by qPCR. The lower Cq values for the engineered qScript Ultra reverse transcriptase indicate more efficient cDNA synthesis at high temperatures up to 65°C, with no loss in performance up to 60°C, critical for progressing through high GC-regions and regions of RNA secondary structure.

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Multiplexing (more than 3 targets)

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  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

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