5PRIME HotMaster Taq DNA Polymerase

Features & Benefits

  • Minimal optimization with self-adjusting magnesium buffer technology
  • Novel HotMaster hot-start/cold-stop technology releases full activity at higher temperatures without activation step
  • Convenient reaction set-up at ambient temperature

 

5PRIME HotMaster Taq is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Hot Start is a well established and convenient method to improve specificity of PCR. However, conventional technologies like antibody mediated inhibition or chemical blocking of DNA polymerases carry a few limitations: long initial activation steps may be required, the performance of the DNA polymerase can be reduced or specificity control may be compromised.

The 5 PRIME HotMaster Taq DNA Polymerase uses an innovative technology to achieve Hot Start PCR. A thermostable inhibitor of the Taq DNA Polymerase is released at high temperatures, thereby immediately activating the enzyme. The HotMaster technology not only provides Hot Start control at reaction setup, but also Cold Stop during the annealing step of each and every cycle of PCR. The result is a unique Hot Start/Cold Stop technology.

Details

  • Contents
    HotMaster Taq DNA Polymerase ( 5U/uL) plus 10x HotMaster Taq Buffer
  • Storage & Handling
    The HotMaster Taq DNA Polymerase kits are shipped on dry ice and should be stored immediately upon receipt at –25°C to -15°C in a constant-temperature freezer. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
  • Related Resources
    CofA (PSF)
    Product Manuals
    Publications
    Genomic Characterization of Crimean–Congo Hemorrhagic Fever Virus in Hyalomma Tick from Spain, 2014
    Maria N.B. Cajimat, Vector-Borne and Zoonotic Diseases - 2017
    Abstract
    Crimean–Congo hemorrhagic fever (CCHF) is a severe tick-borne disease caused by CCHF virus (CCHFV). Ticks in the genus Hyalomma are the main vectors and reservoirs of CCHFV. In Spain, CCHFV was first detected in Hyalomma ticks from Cáceres in 2010. Subsequently, two autochthonous CCHF cases were reported in August 2016. In this study, we describe the characterization of the CCHFV genome directly from Hyalomma lusitanicum collected in Cáceres in 2014. Phylogenetic analyses reveal a close relationship with clade III strains from West Africa, with an estimated divergence time of 50 years. The results of this work suggest that CCHFV has been circulating in Spain for some time, and most likely originated from West Africa.
    Rapid viral symbiogenesis via changes in parasitoid wasp genome architecture
    Gaelen R. Burke, Molecular Biology and Evolution - 2018
    Abstract
    Viral genome integration provides a complex route to biological innovation that has rarely but repeatedly occurred in one of the most diverse lineages of organisms on the planet, parasitoid wasps. We describe a novel endogenous virus in braconid wasps derived from pathogenic alphanudiviruses. Limited to a subset of the genus Fopius, this recent acquisition allows an unprecedented opportunity to examine early endogenization events. Massive amounts of virus-like particles (VLPs) are produced in wasp ovaries. Unlike most endogenous viruses of parasitoid wasps, the VLPs do not contain DNA, translating to major differences in parasitism-promoting strategies. Rapid changes include genomic rearrangement, loss of DNA processing proteins, and wasp control of viral gene expression. These events precede the full development of tissue-specific viral gene expression observed in older associations. These data indicate that viral endogenization can rapidly result in functional and evolutionary changes associated with genomic novelty and adaptation in parasitoids.
    InDel markers for monitoring the introgression of downy mildew resistance from wild relatives into grape varieties
    Serena Foria, Molecular Breeding - 2018
    Abstract
    We identified haplotype-tagging insertion/deletions (InDels) for downy mildew resistance (Rpv3-1) in grapevine and converted them into InDel markers. InDel-25,941 and InDel-26,032 were validated by fragment analysis via capillary electrophoresis in 174 varieties of Vitis vinifera, 50 resistant varieties of the ‘Seibel 4614’ lineage that share Rpv3-1 by descent, and in 83 Vitis accessions. Amplicon sequencing of ancestral and derived alleles revealed that both mutations were caused by deletions. The 25,941-deletion is most likely recent. The derived allele is present only in resistant varieties obtained from ‘Seibel 4614’ and has originated in North American populations through two successive deletions within a predicted multiple stem-loop ssDNA structure, consisting of three nearby short inverted repeats, which shortened the ancestral DNA stepwise. The 26,032-deletion is more ancient. The derived allele is always present in resistant varieties of the ‘Seibel 4614’ lineage, completely absent from V. vinifera, not found in other North American accessions, and rarely present in Asian species. It may have originated in a common ancestral population before the continental disjunction, followed by incomplete lineage sorting, or in either lineage followed by introgression via secondary contacts. Genotyping with these markers does not require special instruments or chemistry for routine screening in breeding practice. Differences in amplicon size between grapes that carry or do not carry Rpv3-1 are detectable via standard agarose gel electrophoresis, or classical melting curve analysis using nonsaturating fluorescent dyes. The recombination rate between each marker and the trait locus is 0.118% for InDel-25,941 and 0.071% for InDel-26,032.
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