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Conventional PCR

Conventional PCR

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.

Quantabio PCR reagents are formulated with ultrapure AccuStart DNA polymerase, which contains a stringent antibody hotstart to ensures specific and efficient primer extension only after activation at 94°C. Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.

PCR comparison chart

	Standard PCR			High Fidelity PCR	Tough PCR	HiFi & Tough PCR	Long PCR
	5PRIME HotMasterMix	AccuStart II PCR SuperMix	AccuStart II GelTrack PCR SuperMix	sparQ HiFi PCR Master Mix	AccuStart II PCR ToughMix	repliQa HiFi ToughMix	AccuStart Long Range SuperMix
Features
Concentration	2.5x	2x	2x	2x	2x	2x	4x
Amplicon Size	Up to 5 kb	Up to 4 kb	Up to 4 kb	Up to 4 kb	Up to 4 kb	Up to 24 kb	Up to 24 kb
Extension Time*	20-60 sec/kb	60 sec/kb	60 sec/kb	30 sec/kb	60 sec/kb	1-10 sec/kb	30-60 sec/kb
Multiplex PCR							Up to 6 Targets
Fidelity vs. Taq	1x	1x	1x	80x Taq	1x	90x Taq	10-12x Taq
dU Tolerant	No	No	No	No	No	Yes	Yes
5’ → 3’ exo	•	•	•	N/A	•	N/A	N/A
3’ → 5’ exo	N/A	N/A	N/A	•	N/A	•	•
Resulting Ends	3’ dA overhang	3’ dA overhang	3’ dA overhang	Blunt	3’ dA overhang	Blunt	Blunt/T overhangs
Applications
Routine PCR	•	•	•		•	•	•
High Fidelity				•		**	•
Crude PCR					•	**
Fast PCR						**
Long PCR						•	**
High Yield		•	•			**	•
NGS Library Amplification				**		•
Available Formats
Hot Start	•	•	•	•	•	•	•
Master Mix	•	•	•	•	•	•	•
Loading Dye Available			•		•
Packaging (Rxns/Units)	100 Rxns 1000 Rxns	100 Rxns 500 Rxns 4000 Rxns	100 Rxns 500 Rxns 4000 Rxns	50 Rxns 250 Rxns	100 Rxns 800 Rxns 4000 Rxns	25 Rxns 100 Rxns 500 Rxns	25 Rxns 100 Rxns

*Range based on amplicon size
**Recommended product

Conventional PCR

PCR comparison chart

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