Conventional PCR

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.

Quantabio PCR reagents are formulated with ultrapure AccuStart DNA polymerase, which contains a stringent antibody hotstart to ensures specific and efficient primer extension only after activation at 94°C.  Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.

PCR comparison chart

  Standard PCR High Fidelity PCR Tough PCR HiFi & Tough PCR Long PCR
  5PRIME HotMasterMix AccuStart II PCR SuperMix AccuStart II GelTrack PCR SuperMix sparQ HiFi PCR Master Mix AccuStart II PCR ToughMix repliQa HiFi ToughMix AccuStart Long Range SuperMix
Features
Concentration 2.5x 2x 2x 2x 2x 2x 4x
Amplicon Size Up to 5 kb Up to 4 kb Up to 4 kb Up to 4 kb Up to 4 kb Up to 24 kb Up to 24 kb
Extension Time* 20-60 sec/kb 60 sec/kb 60 sec/kb 30 sec/kb 60 sec/kb 1-10 sec/kb 30-60 sec/kb
Multiplex PCR Up to 6 Targets
Fidelity vs. Taq 1x 1x 1x 80x Taq 1x 90x Taq 10-12x Taq
dU Tolerant No No No No No Yes Yes
5’ → 3’ exo N/A N/A N/A
3’ → 5’ exo N/A N/A N/A N/A
Resulting Ends 3’ dA overhang 3’ dA overhang 3’ dA overhang Blunt 3’ dA overhang Blunt Blunt/T overhangs
Applications
Routine PCR
High Fidelity **
Crude PCR **
Fast PCR **
Long PCR **
High Yield **
NGS Library Amplification **
Available Formats
Hot Start
Master Mix
Loading Dye Available
Packaging (Rxns/Units) 100 Rxns
1000 Rxns
100 Rxns
500 Rxns
4000 Rxns
100 Rxns
500 Rxns
4000 Rxns
50 Rxns
250 Rxns
100 Rxns
800 Rxns
4000 Rxns
25 Rxns
100 Rxns
500 Rxns
25 Rxns
100 Rxns

*Range based on amplicon size
**Recommended product

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