Conventional PCR
Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.
Quantabio PCR reagents are formulated with ultrapure AccuStart DNA polymerase, which contains a stringent antibody hotstart to ensure specific and efficient primer extension only after activation at 94°C. Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.
Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.
PCR comparison chart
| Standard PCR | High Fidelity PCR | Tough PCR | HiFi & Tough PCR | Long PCR | ||||
|---|---|---|---|---|---|---|---|---|
| 5PRIME HotMasterMix | AccuStart II PCR SuperMix | AccuStart II GelTrack PCR SuperMix | sparQ HiFi PCR Master Mix | AccuStart II PCR ToughMix | repliQa HiFi ToughMix | AccuStart Long Range SuperMix | ||
| Features | ||||||||
| Concentration | 2.5x | 2x | 2x | 2x | 2x | 2x | 4x | |
| Amplicon Size | Up to 5 kb | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 4 kb | Up to 24 kb | Up to 24 kb | |
| Extension Time* | 20-60 sec/kb | 60 sec/kb | 60 sec/kb | 30 sec/kb | 60 sec/kb | 1-10 sec/kb | 30-60 sec/kb | |
| Multiplex PCR | Up to 6 Targets | |||||||
| Fidelity vs. Taq | 1x | 1x | 1x | 80x Taq | 1x | 90x Taq | 10-12x Taq | |
| dU Tolerant | No | No | No | No | No | Yes | Yes | |
| 5’ → 3’ exo | • | • | • | N/A | • | N/A | N/A | |
| 3’ → 5’ exo | N/A | N/A | N/A | • | N/A | • | • | |
| Resulting Ends | 3’ dA overhang | 3’ dA overhang | 3’ dA overhang | Blunt | 3’ dA overhang | Blunt | Blunt/T overhangs | |
| Applications | ||||||||
| Routine PCR | • | • | • | • | • | • | ||
| High Fidelity | • | ** | • | |||||
| Crude PCR | • | ** | ||||||
| Fast PCR | ** | |||||||
| Long PCR | • | ** | ||||||
| High Yield | • | • | ** | • | ||||
| NGS Library Amplification | ** | • | ||||||
| Available Formats | ||||||||
| Hot Start | • | • | • | • | • | • | • | |
| Master Mix | • | • | • | • | • | • | • | |
| Loading Dye Available | • | • | ||||||
| Packaging (Rxns/Units) | 100 Rxns 1000 Rxns |
100 Rxns 500 Rxns 4000 Rxns |
100 Rxns 500 Rxns 4000 Rxns |
50 Rxns 250 Rxns |
100 Rxns 800 Rxns 4000 Rxns |
25 Rxns 100 Rxns 500 Rxns |
25 Rxns 100 Rxns |
|
*Range based on amplicon size
**Recommended product
Featured Products
-
repliQa HiFi ToughMix
Superior speed and inhibitor tolerance -
AccuStart Long Range SuperMix
Superior sensitivity and multiplexing for DNA amplification of long targets -
AccuStart II GelTrack PCR SuperMix
AccuStart II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. Premixed with electrophoretic dye for gel electrophoresis. -
5PRIME HotMasterMix
Available until end of December 2020. Please order bulk quantities if needed.
Highly specific amplification with minimal handling -
5PRIME HotMaster Taq DNA PolymeraseAvailable until end of December 2020. Please order bulk quantities if needed.
Innovative Hot-Start/Cold-Stop technology for highly specific Hot-Start PCR without enzyme activation -
AccuStart II PCR ToughMix
A stabilized, ready-to-use (2X) reaction cocktail for sensitive PCR amplification of DNA templates using crude extracts, clinical specimens, plant tissues, animal tissues, environmental samples and complex food matrices -
AccuStart II Taq DNA Polymerase
Ultrapure, high-yielding Taq DNA polymerase mutant with stringent antibody hot start. -
AccuStart II PCR SuperMix
A stabilized, ready-to-use (2X) reaction cocktail for robust general purpose PCR amplification of DNA fragments up to 4 kb. -
AccuStart Taq DNA Polymerase HiFi
Contains 3'-exonuclease (proof-reading) polymerase for robust amplification of large, complex DNA fragments up to 20 kb in length with reliable sequence fidelity
