“Very good product for NGS library purification and size selection”
sparQ PureMag Beads
Features & Benefits
- High recovery of DNA fragments greater than 100 bp
- Efficient removal of unwanted components from adapter ligation and PCR reactions
- Consistent single or double-sided size selection
- Seamless integration into existing NGS workflows with little or no protocol change
- Easy-to-use and compatible with manual processing or automated liquid handling robots
- Cost effective alternative to AMPure® XP with equivalent performance
Description
Workflow for Double-Sided Size Selection
Workflow for Double-Sided Size Selection

Workflow for Double-Sided Size Selection
Double-sided size selection is used to remove smaller and larger fragments from either side of the desired region. The fragment size can be easily adjusted to suit the application by manipulating the sparQ PureMag Beads to DNA volumetric ratio.
- Double-sided size selection tunable for specific application
- Achieve consistent size range by simply manipulating the beads to sample ratio
Performance Data
Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection

Bioanalyzer trace of fragmented human genomic DNA pre- and post double-sided size selection
Electropherogram of fragmented human genomic DNA pre- and post double-sided size selection. Different sparQ PureMag Beads to DNA ratios were used to achieve various targeted size range.
Highly reproducible purification across a range of inputs

Highly reproducible purification across a range of inputs
Highly reproducible DNA library profiles were achieved using different lots of sparQ PureMag Beads and a broad range of input amount. Libraries were prepared with sparQ DNA Library Prep Kit from 100 ng and 1 ng of fragmented microbial genomic DNA. sparQ PureMag Beads were used post adapter ligation and PCR amplification to effectively remove adapter-dimers and primer-dimers.
Efficient recovery of DNA equivalent to AMPure XP

Efficient recovery of DNA equivalent to AMPure XP
sparQ PureMag Beads show equivalent performance to AMPure XP for DNA purification. 50 bp DNA ladder was purified with sparQ PureMag Beads and AMPure XP at different beads to DNA ratios and analyzed on 2% agarose gel.
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sparQ PureMag Beads