sparQ DNA Frag & Library Prep Kit

Details

• Contents

  sparQ DNA Frag & Library Prep Kit contains:

  • DNA Frag & Polishing Enzyme Mix (5X)
  • DNA Frag & Polishing Buffer (10X)
  • DNA Frag Enhancer Solution
  • DNA Ligase
  • DNA Rapid Ligation Buffer
  • HiFi PCR Master Mix (2X)
  • Primer Mix
• Storage & Handling
  Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
• Related Resources
  Product Flyers

  Flyer sparQ DNA Frag & Library Prep Kit

  Product Manuals

  sparQ DNA Frag & Library Prep Kit

  Technical Notes

  Streamlined Single-Tube Solutions for High Quality DNA Library PreparationOptimization GuideSetting up sparQ Library Prep Kit in Illumina Experiment Manager

  Publications

  Complete Genomes of Symbiotic Cyanobacteria Clarify the Evolution of Vanadium-Nitrogenase

  Jessica M. Nelson, Genome Biology and Evolution - 2019

  Abstract

  Plant endosymbiosis with nitrogen-fixing cyanobacteria has independently evolved in diverse plant lineages, offering a unique window to study the evolution and genetics of plant–microbe interaction. However, very few complete genomes exist for plant cyanobionts, and therefore little is known about their genomic and functional diversity. Here, we present four complete genomes of cyanobacteria isolated from bryophytes. Nanopore long-read sequencing allowed us to obtain circular contigs for all the main chromosomes and most of the plasmids. We found that despite having a low 16S rRNA sequence divergence, the four isolates exhibit considerable genome reorganizations and variation in gene content. Furthermore, three of the four isolates possess genes encoding vanadium (V)-nitrogenase (vnf), which is uncommon among diazotrophs and has not been previously reported in plant cyanobionts. In two cases, the vnf genes were found on plasmids, implying possible plasmid-mediated horizontal gene transfers. Comparative genomic analysis of vnf-containing cyanobacteria further identified a conserved gene cluster. Many genes in this cluster have not been functionally characterized and would be promising candidates for future studies to elucidate V-nitrogenase function and regulation.

  Microbial mitigation of greenhouse gas emissions from boreal lakes

  Gaëtan Martin, Scientific Data - 2021

  Abstract

  The climate change crisis has drawn the attention of both the public and scientific community to the carbon cycle and particularly to the importance of greenhouse gases (GHG) carbon dioxide (CO2) and methane (CH4). CO2 has been a key component of Earth´s climate regulation throughout its geological history and is now the main driver of the current change in climate. CH4 has been responsible for a quarter of the cumulative radiative forcing observed so far. Recent studies suggest that lakes could be a major source of both CO2 and CH4. Boreal lakes are of special interest as they represent 27% of the global lake area, and their production of CO2 and CH4 are expected to increase in the future. This project aimed to investigate microbial processes with the potential to limit the emissions of GHGs from boreal lakes. For that purpose, the impact of an increase in phosphorus (P) concentration in the water on CH4 oxidation under the ice was investigated as well as the community composition of the methanotrophic guild. We also looked at the potential importance of chemolithoautotrophic microorganisms in fixing CO2 in the water column. Using a combination of geochemical analysis, genomic studies, and in vivo assays, we showed that P amendment has the potential to increase methane oxidation, possibly limiting the expected increase in CH4 emissions due to anthropogenic fertilization of boreal lakes. We also showed that methanotrophic community structure in boreal lakes is driven by CH4 concentration and that alphaproteobacterial methanotrophs might play an important role in removing CH4 from surface waters. Finally, we showed that dark carbon fixation is a common trait in boreal lakes and that it seems related to the iron cycle.

  ISSRseq: an extensible, low-cost, and efficient method for reduced representation sequencing

  Brandon T. Sinn, bioRxiv - 2020

  Abstract

  1. The capability to generate densely sampled single nucleotide polymorphism (SNP) data is essential in diverse subdisciplines of biology, including crop breeding, pathology, forensics, forestry, ecology, evolution, and conservation. However, access to the expensive equipment and bioinformatics infrastructure required for genome-scale sequencing is still a limiting factor in the developing world and for institutions with limited resources. 2. Here we present ISSRseq, a PCR-based method for reduced representation of genomic variation using simple sequence repeats as priming sites to sequence inter-simple sequence repeat (ISSR) regions. Briefly, ISSR regions are amplified with single primers, pooled, and used to construct sequencing libraries with a low-cost, efficient commercial kit, and sequenced on the Illumina platform. We also present a flexible bioinformatic pipeline that assembles ISSR loci, calls and hard filters variants, outputs data matrices in common formats, and conducts population analyses using R. 3. Using three angiosperm species as case studies, we demonstrate that ISSRseq is highly repeatable, necessitates only simple wet-lab skills and commonplace instrumentation, is flexible in terms of the number of single primers used, is low-cost, and can generate genomic-scale variant discovery on par with existing RRS methods that require high sample integrity and concentration. 4. ISSRseq represents a straightforward approach to SNP genotyping in any organism, and we predict that this method will be particularly useful for those studying population genomics and phylogeography of non-model organisms. Furthermore, the ease of ISSRseq relative to other RRS methods should prove useful for those conducting research in undergraduate and graduate environments, and more broadly by those lacking access to expensive instrumentation or expertise in bioinformatics.

  Comprehensive dataset of shotgun metagenomes from stratified freshwater lakes and ponds

  Moritz Buck, bioRxiv - 2020

  Abstract

  Stratified lakes and ponds featuring steep oxygen gradients are significant net sources of greenhouse gases and hotspots in the carbon cycle. Despite their significant biogeochemical roles, the microbial communities, especially in the oxygen depleted compartments, are poorly known. Here, we present a comprehensive dataset including 267 shotgun metagenomes from 41 stratified lakes and ponds mainly located in the boreal and subarctic regions, but also including one tropical reservoir and one temperate lake. For most lakes and ponds, the data includes a vertical sample set spanning from the oxic surface to the anoxic bottom layer. The majority of the samples were collected during the open water period, but also a total of 29 samples were collected from under the ice. In addition to the metagenomic sequences, the dataset includes environmental variables for the samples, such as oxygen, nutrient and organic carbon concentrations. The dataset is ideal for further exploring the microbial taxonomic and functional diversity in freshwater environments and potential climate change impacts on the functioning of these ecosystems.

  Click here to see all Publications

  Safety Data Sheets (SDS)

  sparQ DNA Frag & Library Prep Kit SDS - DNA Frag & Polishing Enzyme Mix (5X)sparQ DNA Frag & Library Prep Kit SDS- DNA Frag & Polishing Buffer (10X)sparQ DNA Frag & Library Prep Kit SDS -DNA Frag Enhancer SolutionsparQ DNA Frag & Library Prep Kit SDS -T4 DNA LigasesparQ DNA Frag & Library Prep Kit SDS -DNA Ligation BuffersparQ DNA Frag & Library Prep Kit SDS -2x HiFi PCR Master MixsparQ DNA Frag & Library Prep Kit SDS -Primer Mix

  CofA (PSF)

  PSF-95194-024-Lot#026427PSF-95194-024-Lot#027779PSF-95194-096-Lot#028351PSF-95194-096-Lot#028691PSF-95194-024-Lot#029804PSF-95194-096-Lot#029923PSF-95194-024-LOT#66141494PSF-95194-096-LOT#66141495PSF-951941-024-LOT#66141491PSF-95194-096-LOT#66151999PSF_95141-250-lot#66146465PSF-95194-024-LOT#66157873PSF-95194-096-LOT#66157881PSF-95200-025 Lot#66157897 PSFPSF-95194-024-Lot#66169250PSF-95194-096-Lot#66160019PSF-95194-096-Lot#66169260PSF-95211-096-Lot#66174283PSF-95194-024-LOT#66189523PSF-95194-096-LOT#66185033
• Testimonials
  The sparQ kit safeguards samples from over fragmentation and routinely provides more consistent fragment size. Once optimized, I had peace of mind knowing my samples were safe when I got busy in the lab.”

  Scientist, Laboratory Manager

  Agricultural Genomics Institute

  "I have built >100 libraries with this kit and the results are great."

  Genomics Core Director/Manager

  West Virginia University

Ordering Information

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• sparQ DNA Frag & Library Prep Kit

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  24 rxns

  Order (95194-024)

  96 rxns

  Order (95194-096)
• sparQ UDI Adapters (1-96)
  1 - 96

  Order (95211-096)

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