The sparQ kit safeguards samples from over fragmentation and routinely provides more consistent fragment size. Once optimized, I had peace of mind knowing my samples were safe when I got busy in the lab.”
sparQ DNA Frag & Library Prep Kit
Integrated enzymatic fragmentation and library prep with unrivaled speed and performance
Features & Benefits
- High quality libraries in 2.5 hours from 1 ng – 1 μg of input DNA
- Tunable and reproducible fragmentation size range
- Simple, convenient 2-step workflow with minimal hands-on time
- Novel chemistry and high-fidelity amplification minimizing bias
- Superior sequence coverage uniformity and low duplication rate
sparQ DNA Frag & Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Description
The sparQ DNA Frag & Library Prep Kit is optimized for enzymatic fragmentation of DNA and streamlined construction of high quality libraries for sequencing on Illumina NGS® platforms. The simple, convenient 2-step workflow can be completed in 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 1 ng to 1000 ng. The DNA fragmentation and polishing reactions are combined in a single step to generate 5'-phosphorylated and 3'-dA-tailed fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-Free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.
Streamlined workflow
sparQ DNA Frag & Library Prep workflow
sparQ DNA Frag & Library Prep workflow
The streamlined workflow utilizes a proprietary enzyme mix that combines fragmentation and DNA polishing in a single step to simplify library construction. High efficiency adapter ligation and cleanup are performed in the same tube, followed by an optional amplification step using HiFi PCR Master Mix and Primer Mix.
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- Generates high quality libraries in 2.5 hours with minimal hands-on time
- Simplified 2-step workflow minimizes sample loss
Tunable & reproducible fragmentation
Fragmentation time course
Fragmentation time course
sparQ DNA Frag & Library Prep Kit is tunable to the desired fragment size. 100 ng Human gDNA was subjected to fragmentation with a series of incubation time points (3 – 18 min). After fragmentation, DNA samples were purified and then visualized using Agilent High Sensitivity DNA Kit.
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- Fragmentation profile closely resembles Covaris mechanical shearing
- Proprietary frag and polishing enzyme mix ensures tunable and reproducible fragmentation across a wide range of DNA input
High coverage uniformity
Genome coverage analysis
Genome coverage analysis
Library prepared using sparQ DNA Frag & Library Prep Kit resulted in uniform coverage across a wide range of GC-content. Libraries were prepared using different DNA fragmentation and library preparation kits with 1 ng or 100 ng of microbial genomic DNA followed by sequencing on Illumina MiSeq. Libraries prepared using PCR-free workflow of sparQ DNA Frag & Library Prep Kit with 100 ng of microbial genomic DNA shows similar performance as a typical amplified library prepared by Covaris mechanical shearing method.
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- Maintains even coverage across a broad GC-spectrum and a wide range of input level
- Low amplification bias results in PCR-free like coverage uniformity
High quality sequencing metrics
High quality sequencing metrics
High quality sequencing metrics
sparQ DNA Frag & Library Prep Kit generates high quality DNA libraries with minimal duplication artifacts. Libraries were prepared with 1 ng and 100 ng of microbial genomic DNA, amplified for 12 and 6 cycles respectively, and subsequently sequenced on Illumina MiSeq. Each library was down-sampled to 2 million reads (150 bp paired-end reads) and aligned to a reference genome with only unique alignments reported.
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- High mapping percentage and low duplication artifacts
- Industry leading sequencing results ensures better return on investment
sparQ Adapter Barcode Sets
sparQ Adapter Barcode Sets A and B are single-indexed (or barcoded) oligonucleotide adapters designed for use with the Quantabio sparQ DNA Library Prep Kits in the construction of libraries for Illumina NGS platforms. Each sparQ Adapter Barcode Set contains 12 single index barcodes for 96 reactions.
| sparQ Adapter Barcode Set A | |
| Barcode Number | Barcode Sequence |
| 2 | CGATGT |
| 4 | TGACCA |
| 5 | ACAGTG |
| 6 | GCCAAT |
| 7 | CAGATC |
| 12 | CTTGTA |
| 13 | AGTCAA |
| 14 | AGTTCC |
| 15 | ATGTCA |
| 16 | CCGTCC |
| 18 | GTCCGC |
| 19 | GTGAAA |
| sparQ Adapter Barcode Set B | ||
| Barcode Number | Barcode Sequence | |
| 1 | ATCACG | |
| 3 | TTAGGC | |
| 8 | ACTTGA | |
| 9 | GATCAG | |
| 10 | TAGCTT | |
| 11 | GGCTAC | |
| 20 | GTGGCC | |
| 21 | GTTTCG | |
| 22 | CGTACG | |
| 23 | GAGTGG | |
| 25 | ACTGAT | |
| 27 | ATTCCT | |
sparQ NGS Library Prep Solutions
Streamlined single-tube solutions for high quality DNA library preparation
What Customers Say
The sparQ kit safeguards samples from over fragmentation and routinely provides more consistent fragment size. Once optimized, I had peace of mind knowing my...
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Scientist, Laboratory Manager
Agricultural genomics institute
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sparQ DNA Frag & Library Prep Kit
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sparQ Adapter Barcode Set A12 single index barcodes for 96 rxns
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sparQ Adapter Barcodes Set B12 single index barcodes for 96 rxns
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