PerfeCTa SYBR® Green SuperMix

Sensitive and precise DNA amplification with DNA-intercalating dye based detection chemistry

Features & Benefits

  • 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy
  • Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start
  • Supports efficient vortex mixing with proprietary anti-foaming formulation
  • SuperMix version provides maximum dye concentration for robust optical signal with small amplicons (i.e. microRNAtemplated cDNA)

 

PerfeCTa SYBR Green SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

PerfeCTa SYBR Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). Successful detection with a non-specific, dsDNA intercalating dye requires precise target amplification as off-target primer elongation will contribute to overall fluorescent signal and lead to over-reported relative abundance values. This reagent is powered by a highly-processive, ultra-pure Taq DNA polymerase mutant with stringent, ultra-pure AccuStart™II antibody hot start technology that allows ambient room-temperature setup and maximal enzyme kinetics after rapid, irreversible denaturation at 95°C.

Performance Data

Comparison to QuantiTect, GAPD


PerfeCTa SYBR Green SuperMix Comparison to QuantiTect SYBR Green PCR Kit (Qiagen), GAPD

Log-fold serial dilutions of qScript™ cDNA from HeLa cell total RNA (100 ng to 0.1 pg) were analyzed by SYBR Green qPCR following each manufacturers protocol. Averaged plots of triplicate qPCR reactions are shown.


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