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PerfeCTa qPCR ToughMix

Exceptional Stability for Tough-Tested real-time PCR

Features & Benefits
  • ToughMix additives neutralize PCR inhibitors to ensure reliable assay performance with a spectrum of starting materials including: clinical specimens, plants, soil, environmental or complex food matrices
  • Superior assay sensitivity and target precision with AccuStart™ II enzyme technology – maximum-yielding, engineered Taq DNA polymerase with stringent, ultrapure antibody hotstart
  • Easy-to-use 2X concentrated master mixes with AccuVue™ plate loading dye and optimized passive reference dye for simplified reaction setup
  • Supports efficient vortex mixing with proprietary anti-foaming technology
  • Consistent and reliable performance with exceptional reagent stability (uninhibited after 20X freeze-thaw or 30 days at 22°C)

 

PerfeCTa qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Instrument compatibility 

PerfeCTa qPCR ToughMix – ROX

 

Request Sample

 

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7300
  • Applied Biosystems 7900
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOnePlus™

PerfeCTa qPCR ToughMix Low-ROX

 

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Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®

PerfeCTa qPCR ToughMix 

 

Request Sample

 

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other

 

PerfeCTa qPCR ToughMix is available in formulations containing UNG (uracil-N-glycosylase). To learn more or request a sample, visit the product page

What Scientists are Saying
PerfeCTa qPCR ToughMix

"Higher sensitivity, positives at a lower Cq. Great results with plant DNA with high inhibition level."

Paula S. | Lab Coordinator INIAV
Q RT-PCR machine in Mbabane Swaziland
We had the pleasure of testing out the ToughMix Mastermix and the “Q” RT-PCR machine in Mbabane Swaziland in our Tuberculosis Centre of Excellence.” As it’s name implies, the ToughMix stood up to its name and made the 38 hour trip from Houston to Mbabane, Swaziland and had beautiful Ct curves. Similarly, the Q PCR machine was an ease to travel with as carry on luggage. It’s 25 minute run gave Ct curves identical to our existing, and much bulkier, RT-PCR machine that was already on site in Swaziland. Considering it’s size, intuitive software, and near perfect reproducible Ct curves, we’ve decided to add the Q as our workhorse RT-PCR machine
Assistant Professor | Infectious Diseases Global and Immigrant Health Baylor College of Medicine
PerfeCTa qPCR ToughMix

"This eliminates a costly and time consuming step to remove inhibitors manually, so it has saved us money on reagents and time for our small lab."

Max O. | Conservationist

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

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