PerfeCTa Multiplex qPCR ToughMix

Advanced 1-tube SuperMix optimized to support highly multiplexed DNA amplifications in miniaturized reaction volumes and withstand a broad spectrum of PCR inhibitors

Features & Benefits

  • 1-tube SuperMix minimizes pipetting steps, simplifying reaction assembly and improving accuracy
  • 5x concentrated reagent provides more flexibility with dilute DNA samples sensitivity
  • ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
  • Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
  • Supports efficient vortex mixing with proprietary anti-foaming additives


PerfeCTa Multiplex qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.


Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix transcends these limitations by enabling sensitive, broad linear dynamic detection range with co-amplification of four abundant (106) targets. PerfeCTa MultiPlex qPCR ToughMix enables multiplex qPCR assay performance comparable to single-plex qPCR probe assays without the need to rigorously titrate primer concentration. PerfeCTa MultiPlex qPCR ToughMix is a 5x concentrated, ready to-use reaction cocktail for real-time quantitative PCR (qPCR) with ToughMix reagent technology that neutralizes a broad spectrum of PCR inhibitors that compromise assay performance with crude extracts, clinical specimens, plant, soil environmental or complex food matrices. A key component of the kit is an ultrapure, highly-processive thermostable DNA polymerase that is free of detectable E. coli DNA. PPerfeCTa MultiPlex qPCR ToughMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples.

Featured Publication

A Multiplexed Real-time PCR Assay to Detect SCID and SMN1 Homozygous Exon 7 Deletion, and A Droplet Digital PCR Assay to Assess SMN2 Copy Numbers, in Newborn screening for Spinal Muscular Atrophy

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