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NEW! UltraPlex 1-Step ToughMix Added to SalivaDirect - Emergency Use Authorization Protocol

SalivaDirectTM EUA Summary

Application Note: Extraction-free COVID-19 detection with Quantabio’s RT-qPCR ToughMixes

UltraPlex 1-Step ToughMix used in direct-lysis saliva SARS-CoV-2 detection

1-Step Virus ToughMix- optimized to detect for low copy inputs in Flu-A, Flu-B and SARS-CoV-2

CDC Adds Two Quantabio RT-qPCR ToughMix Kits to COVID-19 Diagnostic Testing Protocol

qScript XLT 1-Step RT-qPCR ToughMix and UltraPlex 1-Step ToughMix granted Emergency Use Authorization (EUA)

Latest Application Note

UltraPlex® 1-Step ToughMix® for Extraction-free, High Capacity COVID-19 Testing

We are working tirelessly to increase our production capacities as it relates to the following RNA RT-qPCR reagents which are cited in the CDC protocol that has received FDA Emergency Use Authorization (EUA) approval.

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CDC Protocol
 



Quantabio’s UltraPlex 1-Step ToughMix Continues to Enhance CDC Protocols to Detect and Differentiate COVID-19 and Influenza Respiratory Diseases


CDC’s latest emergency use authorization for influenza SARS-CoV-2 assay validates Quantabio’s ability to multiplex up to five targets with high efficiency, sensitivity and specificity BEVERLY, M...

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Quantabio RT-qPCR ToughMix Given Expanded Role in CDC COVID-19 Testing Protocol



Amended CDC emergency use authorization recognizes UltraPlex formulation in an effective heat treatment alternative for RNA extraction BEVERLY, Mass. – July 1, 2020 – Quantabio, a leading provide...

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Quantabio UltraPlex 1-Step ToughMix used for direct-lysis saliva SARS-CoV-2 detection




We evaluated UltraPlex 1-Step ToughMix (4X) in a direct-lysis (no extraction step) qPCR-based SARS-CoV-2 detection protocol from saliva samples. The current protocol uses
Read More We evaluated UltraPlex 1-Step ToughMix (4X) in a direct-lysis (no extraction step) qPCR-based SARS-CoV-2 detection protocol from saliva samples. The current protocol uses TaqPath 1-Step RT-qPCR Master Mix, CG; ToughMix was used as a 1:1 replacement in the current protocol and performed as well as, or better, in each condition tested: clinically-validated positive saliva samples, plasmid copy number detection, and negative saliva samples (test for false positives). We will be switching to ToughMix as our primary enzyme, with TaqPath as the backup in case any supply-chain issues arise.

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Product Comparisons

Determine which RT-qPCR kit is right for your research needs

qScript XLT 1-Step
RT-qPCR ToughMix		qScript 1-Step Virus ToughMixUltraPlex 1-Step ToughMix
Recommended in
CDC protocol
Detection purified RNA
Extraction-free detection
Saliva-direct detection
Formulation 2X master mix 2X master mix 4X master mix
Taq Hot Start Yes Yes Yes
RT Hot Start No Yes Yes
Chemistry 1 tube SuperMix 1 tube SuperMix 1 tube SuperMix
Performance ++++ ++++ ++++
Cycling Conditions Fast or Standard qPCR Cycling Fast or Standard qPCR Cycling Fast or Standard qPCR Cycling
Multiplex Capabilty Up to 4 targets Up to 4 targets Up to 5 targets
Sensitivity +++ ++++ ++++
Inhibitor Tolerance Yes Yes Yes

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