repliQa HiFi Toughmix

repliQa HiFi ToughMix

Superior speed and inhibitor tolerance
Features & Benefits



    • Extreme Speed: up to 3x faster PCR results with extension rates as fast as 1 kb/sec*
    • Long Range: amplify 24+ kb gDNA
    • Tough-tested: tolerant to a wide range of inhibitors

    *for fragments less than 1 kb in size






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    Application Note

    Optimal PCR amplification for GC-rich templates using repliQa HiFi ToughMix and enhancers

    PCR amplification of DNA fragments with high GC-content can be challening and require extensive optimization. In this application note, we present a fast and efficient method to amplify GC-rich templates using repliQa Hifi ToughMix and easily available PCR ehnancers.

    Download Application Note

    What our customers are saying
    repliQa HiFi ToughMix

    "All I can say from testing repliQa HiFi ToughMix is that the results are jaw-dropping. The Platinum™ SuperFi II PCR Master Mix did nothing in the same amount of time under the same conditions. The assay I was testing out is a new way to amplify entire mitogenomes as a single amplicon (~15kb). This is a game changer, cutting the PCR time from ~8.5hrs to 1.5hrs, and way more product is generated. I am not often floored by data, but these results are amazing. I have another project I am working on with this assay and I am going to switch to this polymerase for sure. Good work Quantabio!"

    A. K. | Post Doc, BCM
    repliQa HiFi ToughMix

    "This can amplify the tough template for SURE. I was struggled with the tough template that showed poor quality results with other many PCR enzymes. This repliQa saved my life and eventually gave me the clear and strong band by gel."

    Lili C. | Post Doc, USC
    repliQa HiFi ToughMix

    "The kit have a great power of amplification and extreme fast speed, I just need one hour to get result which I usually spent three hours before."

    Simon S. | Professor, Tennessee State University

    Product Finder

    Select Your Assay

    Starting Template

    Assay Format

    Detection Chemistry

    Multiplexing (more than 3 targets)

    Is gene-specific priming (GSP) required?

    What current Reverse Transcriptase or cDNA kit are you using?

    Select the group which contains your real-time PCR cycler

    • Applied Biosystems 7500
    • Applied Biosystems 7500 Fast
    • Stratagene Mx3000P®
    • Stratagene Mx3005P™
    • Stratagene Mx4000™
    • Applied Biosystems ViiA 7
    • Applied Biosystems QuantStudio™
    • Agilent AriaMx
    • Douglas Scientific IntelliQube®
    • Applied Biosystems 5700
    • Applied Biosystems 7000
    • Applied Biosystems 7300
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    • Applied Biosystems 7900
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    • Applied Biosystems 7900 HT Fast
    • Applied Biosystems StepOne™
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    • Quantabio Q
    • BioRad CFX
    • Roche LightCycler 480
    • QIAGEN Rotor-Gene Q
    • Azure Cielo 6
    • Azure Cielo 3
    • Other
    • BioRad iCycler iQ™
    • BioRad MyiQ™
    • BioRad iQ™5

    Choose your application from the categories below


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