repliQa HiFi ToughMix
- Extreme Speed: up to 3x faster PCR results with extension rates as fast as 1 kb/sec*
- Long Range: amplify 24+ kb gDNA
- Tough-tested: tolerant to a wide range of inhibitors
*for fragments less than 1 kb in size
PCR amplification of DNA fragments with high GC-content can be challening and require extensive optimization. In this application note, we present a fast and efficient method to amplify GC-rich templates using repliQa Hifi ToughMix and easily available PCR ehnancers.
"All I can say from testing repliQa HiFi ToughMix is that the results are jaw-dropping. The Platinum™ SuperFi II PCR Master Mix did nothing in the same amount of time under the same conditions. The assay I was testing out is a new way to amplify entire mitogenomes as a single amplicon (~15kb). This is a game changer, cutting the PCR time from ~8.5hrs to 1.5hrs, and way more product is generated. I am not often floored by data, but these results are amazing. I have another project I am working on with this assay and I am going to switch to this polymerase for sure. Good work Quantabio!"
"This can amplify the tough template for SURE. I was struggled with the tough template that showed poor quality results with other many PCR enzymes. This repliQa saved my life and eventually gave me the clear and strong band by gel."
"The kit have a great power of amplification and extreme fast speed, I just need one hour to get result which I usually spent three hours before."