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repliQa HiFi ToughMix

Superior speed and inhibitor tolerance

Features & Benefits

Extreme Speed: up to 3x faster PCR results with extension rates as fast as 1 kb/sec*
Long Range: amplify 24+ kb gDNA
Tough-tested: tolerant to a wide range of inhibitors

*for fragments less than 1 kb in size

LEARN MORE

Application Note

Optimal PCR amplification for GC-rich templates using repliQa HiFi ToughMix and enhancers

PCR amplification of DNA fragments with high GC-content can be challening and require extensive optimization. In this application note, we present a fast and efficient method to amplify GC-rich templates using repliQa Hifi ToughMix and easily available PCR ehnancers.

Download Application Note

What our customers are saying

repliQa HiFi ToughMix

"All I can say from testing repliQa HiFi ToughMix is that the results are jaw-dropping. The Platinum™ SuperFi II PCR Master Mix did nothing in the same amount of time under the same conditions. The assay I was testing out is a new way to amplify entire mitogenomes as a single amplicon (~15kb). This is a game changer, cutting the PCR time from ~8.5hrs to 1.5hrs, and way more product is generated. I am not often floored by data, but these results are amazing. I have another project I am working on with this assay and I am going to switch to this polymerase for sure. Good work Quantabio!"

A. K. | Post Doc, BCM

repliQa HiFi ToughMix

"This can amplify the tough template for SURE. I was struggled with the tough template that showed poor quality results with other many PCR enzymes. This repliQa saved my life and eventually gave me the clear and strong band by gel."

Lili C. | Post Doc, USC

repliQa HiFi ToughMix

"The kit have a great power of amplification and extreme fast speed, I just need one hour to get result which I usually spent three hours before."

Simon S. | Professor, Tennessee State University

repliQa HiFi ToughMix

Application Note

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

Choose your application from the categories below

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