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ToughMix®
  • Robust fluorescent probe qPCR & PCR performance
  • Applicable to wider range of probe designs and detection chemistries
  • Superior performance with pure template
  • Overcomes many known PCR inhibitors

 

 

Common PCR inhibitors and their source:

InhibitorsCommon Source
Polysaccharides Plants, seeds, stool
Heme, hemoglobin Blood
Humic acid Soil, plant material
Melanin Skin, hair

Popular ToughMix® Products

  • qScript XLT 1-Step RT-qPCR ToughMix A highly sensitive, ready-to-use master mix for quantitative reverse transcription PCR (RT-qPCR) of RNA templates using probe-based detection methods
  • UltraPlex 1-Step ToughMix An advanced 4X concentrated master mix that enables highly sensitive 1-step RNA detection (RT-qPCR) using multiplexed probe-based real-time PCR with crude extracts or inhibitory biological starting materials
  • AccuStart Genotyping ToughMix An advanced 2X concentrated ready-to-use reaction cocktail optimized with destabilizing buffer chemistry to enable accurate SNP genotyping with crude extracts, clinical specimens, environmental samples, plant tissues, animal tissues, or complex food matrices
  • PerfeCTa Multiplex qPCR ToughMix

    An advanced 5X concentrated ToughMix reagent optimized to support highly-multiplexed real-time qPCR amplification across a range of inhibitory sample types and crude extraction methods