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sparQ PureMag Beads

Fast, reliable nucleic acid clean-up & size selection for NGS workflows
Features & Benefits
  • High recovery of DNA fragments greater than 100 bp
  • Efficient removal of unwanted components from adapter ligation and PCR reactions
  • Consistent single or double-sided size selection
  • Seamless integration into existing NGS workflows with little or no protocol change
  • Easy-to-use and compatible with manual processing or automated liquid handling robots
  • Cost effective alternative to AMPure® XP with equivalent performance
Product
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Product
Kit Size
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Price
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sparQ PureMag Beads

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Kit Size:  5 mL
Part Number:  95196-005
Price:  $258.00
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Kit Size:  60 mL
Part Number:  95196-060
Price:  $959.00
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Kit Size:  450 mL
Part Number:  95196-450
Price:  $4,628.00
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Description

sparQ PureMag Beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads, this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag Beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

What Customers Say

Beads performed as well in my tests as Ampure XP which I consider to be the gold standard. Impressive results given the lower price for sparQ PureMag Beads.”

University of Southern California
Director of Sequencing Technology
Details

Details

Contents
Cat. No 95196-005 Size:    5 mL
  95196-060    60 mL
  95196-450 450 mL
Customer Testimonials

Customer Testimonials

sparQ PureMag Beads
I used sparQ PureMag Beads for size selection following directly the Quantabio protocol instructions in terms of ratio beads:chromatin and I got a very precise size selection. I also used these beads for the final NGS library clean-up and worked well as well.
Postdoctoral Fellow | NYU Langone Health
sparQ PureMag Beads

“I used the sparQ PureMag beads for mRNA purification after in vitro transcription. The sparQ beads demonstrated an excellent recovery (~98%) and could be used for size selection of the desired mRNA product. Highly recommended."

Postdoctoral Fellow | Dana Farber Cancer Institute
sparQ PureMag Beads

"Worked exactly as advertised. I was able to get complete recovery of my DNA easily and quickly."

Postdoctoral Fellow | BC CANCER RESEARCH CENTRE
sparQ PureMag Beads

"Very good product for NGS library purification and size selection"

Associate Professor | University of Texas MD Anderson Cancer Center
sparQ PureMag Beads

"Very easy to use and really good recovery yield."

Biologist | BATJ, Inc
sparQ PureMag Beads

"Beads performed as well in my tests as Ampure XP which I consider to be the gold standard.  Impressive results given the lower price for sparQ PureMag Beads."

Director of Sequencing Technology | University of Southern California
sparQ PureMag Beads

"We found that size selection with sparQ PureMag Beads was similar to our current vendor for but the amount of size selected library recovered was significantly larger."

Vice President of Operations | PHASE GENOMICS
sparQ PureMag Beads

"Great product. Useful for many different types of DNA capture applications."

Associate Professor | University of South Carolina

Details

Contents
Cat. No 95196-005 Size:    5 mL
  95196-060    60 mL
  95196-450 450 mL

Performance Data

Resources

Customer Profile Stories

Flyers

Product Manuals

Application Notes

Brochures

CofA (PSFs)

SDSs

FAQs

What is the recovery rate with sparQ PureMag Beads?
The recovery rate depends on many factors, including the fragment size, sample volume, sample concentration, and elution volume. Up to 90% recovery rate can be expected under the optimal condition. The yield is also correlated with the ratio of beads to sample, and the lower ratio usually results in lower yield. When the ratio reaches to 1.8X, it generally produces maximal recovery.
Is there a size limit for the DNA fragments that can be bound with sparQ PureMag Beads?
sparQ PureMag Beads will bind DNA fragments 100 bp and above and exclude primers 50 bases and below. There will be some recovery for oligo or DNA between 50 and 100 bases.
How do I dry my samples before elution?
Air-dry the beads on the magnetic stand for 5 min or until the beads are dry and before they start to crack. Do not over-dry the beads as it can reduce the DNA recovery.
Can I still use the sparQ PureMag Beads if it was accidentally frozen?
sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.
Can I still use the sparQ PureMag Beads if I left it at room temperature for some time?
sparQ PureMag Beads is manufactured and tested for the storage temperature indicated on the bottle and we cannot guarantee performance if it was stored outside of the recommended condition.

Publications

Customer Testimonials

sparQ PureMag Beads
I used sparQ PureMag Beads for size selection following directly the Quantabio protocol instructions in terms of ratio beads:chromatin and I got a very precise size selection. I also used these beads for the final NGS library clean-up and worked well as well.
Postdoctoral Fellow | NYU Langone Health
sparQ PureMag Beads

“I used the sparQ PureMag beads for mRNA purification after in vitro transcription. The sparQ beads demonstrated an excellent recovery (~98%) and could be used for size selection of the desired mRNA product. Highly recommended."

Postdoctoral Fellow | Dana Farber Cancer Institute
sparQ PureMag Beads

"Worked exactly as advertised. I was able to get complete recovery of my DNA easily and quickly."

Postdoctoral Fellow | BC CANCER RESEARCH CENTRE
sparQ PureMag Beads

"Very good product for NGS library purification and size selection"

Associate Professor | University of Texas MD Anderson Cancer Center
sparQ PureMag Beads

"Very easy to use and really good recovery yield."

Biologist | BATJ, Inc
sparQ PureMag Beads

"Beads performed as well in my tests as Ampure XP which I consider to be the gold standard.  Impressive results given the lower price for sparQ PureMag Beads."

Director of Sequencing Technology | University of Southern California
sparQ PureMag Beads

"We found that size selection with sparQ PureMag Beads was similar to our current vendor for but the amount of size selected library recovered was significantly larger."

Vice President of Operations | PHASE GENOMICS
sparQ PureMag Beads

"Great product. Useful for many different types of DNA capture applications."

Associate Professor | University of South Carolina

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