Sensitive first-strand cDNA synthesis of RNA sequences ≤1kb for quantitative and conventional two-step RT-PCR.
Broad linear dynamic detection range with limiting (10pg) or plentiful (4ug) samples of total RNA.
Pre-blended with ribonuclease inhibitor protein (RIP) to prevent RNA template degradation during incubation and a proprietary blend of random and oligo(dT) primers, optimized to deliver unbiased representation of 5’ and 3’ sequences.
qScript XLT cDNA SuperMix
Quantabio Catalog #: 95161-025
Stabilized 5X concentrated master mix simplifies reaction assembly and improves accuracy by minimizing pipetting steps.
Optimized, double pre-primed SuperMix provides equal representation of 5' and 3' RNA sequences ≤ 1kb for quantitative and conventional two-step RT-PCR.
Improved RNA template binding affinity for increased yields with problematic sequences or complex secondary structure.
Perform more qPCR assays from limiting amounts of RNA with 2-3 fold improved cDNA yields over older enzyme technologies.
Broad linear dynamic detection range spanning up to 108 fold.
2nd Step Reagents
PerfeCTa MultiPlex qPCR SuperMix
Quantabio Catalog #: 95063-050
Maximum assay sensitivity and target precision with stringent, ultrapure, and high-yielding AccuStart™ II hot start Taq DNA polymerase.
Broad linear detection range with cDNA (106) or genomic DNA (104) templates.