qScript XLT 1-Step RT-qPCR ToughMix

Tough-tested 1-step real time PCR

Features & Benefits

  • Tough Tested - Overcomes common inhibitors including polysacharides, heme/hemoglobin, humic acid, melanin
  • Flexible - Use fast or standard qPCR cycling conditions
  • Broad dynamic range- Reliable data from your precious samples every time
  • Multiplexing enabled - Supports highly sensitive detection for up to four targets

 

qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5'-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters. This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies. Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
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Details

  • Contents

    2X concentrated One-Step Master Mix containing:

    • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
    • qScript XLT reverse transcriptase
    • RNase inhibitor protein
    • AccuStart II Taq DNA Polymerase
    • Inert AccuVue™ plate loading dye
    • Proprietary enzyme stabilizers and performance-enhancing additives
    • Titrated reference dye (if applicable)
  • Storage & Handling
    qScript XLT One-Step RT-qPCR ToughMix is stable for up to one year when stored in a constant temperature freezer at -20°C protected from light. Repeated freezing and thawing does not affect RT-qPCR performance.
  • Resources
  • Instrument Capability
    High ROX
    • Applied Biosystems 5700
    • Applied Biosystems 7000
    • Applied Biosystems 7300
    • Applied Biosystems 7700
    • Applied Biosystems 7900
    • Applied Biosystems 7900HT
    • Applied Biosystems 7900 HT Fast
    • Applied Biosystems StepOne™
    • Applied Biosystems StepOnePlus™
    Low ROX
    • Applied Biosystems 7500
    • Applied Biosystems 7500 Fast
    • Stratagene Mx3000P®
    • Stratagene Mx3005P™
    • Stratagene Mx4000™
    • Applied Biosystems ViiA 7
    • Applied Biosystems QuantStudio™
    • Agilent AriaMx
    • Douglas Scientific IntelliQube®
    • QIAGEN Rotor-Gene Q
    No ROX
    • BioRad CFX
    • Other
    Bio-Rad iCycler iQ systems
    • BioRad iCycler iQ™
    • BioRad MyiQ™
    • BioRad iQ™5

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