US

qScript XLT 1-Step RT-PCR Kit

Tough-tested One-Step Reverse Transcriptase PCR (RT-PCR) in a simplified, 2-component reagent system.
Features & Benefits
  • ToughMix reagent technology withstands PCR inhibitors commonly found in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
  • Optional GelTrack dye streamlines workflow for gel electrophoresis
  • Temperature stabilized for support reaction assembly at convenient ambient room temperatures
  • Preblended with ribonuclease inhibitor protein to preserve RNA integrity during incubation
  • 3′-exonuclease proof-reading polymerase supports high-fidelity downstream applications

 

qScript XLT 1-Step RT-PCR Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Part Number
Price
Quantity
 
Product
Kit Size
Order Info
Price
Quantity

qScript XLT 1-Step RT-PCR Kit

Request Sample
Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  20 x 25 μL rxns
Part Number:  95143-020
Price:  $85.00
Add to Cart
Kit Size:  200 x 25 μL rxns
Part Number:  95143-200
Price:  $657.00
Add to Cart

Description

The qScript XLT One-Step RT-PCR Kit is a convenient and highly sensitive 2-reagent system for amplification of complex RNA templates up to 4 kb in length. Both enzyme incubation sequences are carried out in the same reaction mixture without opening between procedures. Advanced qScript XLT reverse transcriptase mutant possesses elevated temperature stability and improved template binding affinity for large complex RNA templates. Supports a wide-range of challenging
starting materials with PCR-inhibitor neutralizing ToughMix additives and delivers consistent, reliable assay performance. Ultrapure AccuStart II hot-start Taq DNA polymerase with 3'-exonuclease proof-reading activity provides stringent activation control for sensitive and precise target amplification.

Details

Details

Contents
  • 25X concentrated One-Step RT master mix containing: qScript XLT reverse transcriptase ribonuclease inhibitor protein (RIP).
  • 2X concentrated One-Step HiFi PCR ToughMix containing:
    • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
    • AccuStart II Taq DNA Polymerase with added 3′-exonuclease proofreading activity
    • Proprietary enzyme stabilizers and performance-enhancing additives.
    • Titrated reference dye (if applicable).
  • GelTrack® Loading Dye: 50X concentrated mixture of RT-PCR compatible, blue and yellow electrophoretic mobility dyes. (Does not contain density reagent)
  • Nuclease-free water

Details

Contents
  • 25X concentrated One-Step RT master mix containing: qScript XLT reverse transcriptase ribonuclease inhibitor protein (RIP).
  • 2X concentrated One-Step HiFi PCR ToughMix containing:
    • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
    • AccuStart II Taq DNA Polymerase with added 3′-exonuclease proofreading activity
    • Proprietary enzyme stabilizers and performance-enhancing additives.
    • Titrated reference dye (if applicable).
  • GelTrack® Loading Dye: 50X concentrated mixture of RT-PCR compatible, blue and yellow electrophoretic mobility dyes. (Does not contain density reagent)
  • Nuclease-free water

Resources

Flyers

Product Manuals

Application Notes

CofA (PSFs)

Click here to see all CofA (PSFs)

SDSs

Publications

Welcome to the Quantabio webshop!

Please complete the new user account registration form

Once your account is setup, you'll be able to purchase any of the products on our site at any time with next day delivery for in stock products.

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

I give Quantabio or an authorized Quantabio distributor permission to contact me for product updates and news.
* Required information