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Fast Cycling qRT-PCR

Quanta Biosciences is introducing the first One-Step Fast qRT-PCR kits for fast-
cycling parameters and uncompromised sensitivity and specificity.

qScript™ One-Step qRT-PCR

qScript™ One-Step qRT-PCR kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These highly sensitive kits enable unbiased co-amplification of low copy RNA transcripts within complex target mixtures.

qScript™ One-Step qRT-PCR kits are available and optimized for all real-time PCR instrument platforms including those requiring normalization with ROX reference dye. See our Selection Guide for TaqMan Probe Detection or go directly to the corresponding qRT-PCR product list.

• Sensitivity - unbiased representation of all RNA targets regardless of abundance
• Efficiency - proprietary reaction buffer maximizes activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase
• Duplex - Unbiased duplexing of low copy RNA transcripts and reference genes within complex target mixtures
• High Fluorescent signal- optimized for TaqMan® and TaqMan® MGB™ probes

Performance

qScript™ One-Step Fast qRT-PCR Kit

Rapid One-Step qRT-PCR with Broad Dynamic Range and High Sensitivity (ACTB)
Beta actin mRNA was amplified from log-fold serial dilutions of HeLa cell total RNA (1 µg to 1 pg) in 10-µL volumes with the qScript™ One-Step Fast qRT-PCR Kit and FAM-BHQ1 labeled 5’-hydrolysis probe. Triplicate reactions for each RNA quantity were incubated for 5 min at 50ºC followed by 30s at 95ºC and then 40 cycles of 3s, 95ºC; 20s, 60ºC on a Mastercycler® ep realplex S (Eppendorf). Total run time was under 41 minutes.

More Performance Data

• qScript™ One-Step Fast qRT-PCR Kit Comparison to QuantiFast™ Probe RT-PCR Kit (Qiagen)
• qScript™ One-Step Fast qRT-PCR Kit Comparison to SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)
• qScript™ One-Step Fast qRT-PCR Kit Comparison to TaqMan® One-Step RT-PCR Reagents (Applied Biosystems)
• qScript™ One-Step Fast qRT-PCR Kit Comparison to iScript™ One-Step RT-PCR Kit for Probes (Bio-Rad)

qScript™ One-Step Fast MGB qRT-PCR Kit

Rapid One-Step qRT-PCR with Broad Dynamic Range and High Sensitivity (GAPD)
Glyceraldehyde-3-phosphate dehydrogenase mRNA was amplified from log-fold serial dilutions of HeLa cell total RNA (1 µg to 1 pg) in 10-µL volumes with the qScript™ One-Step Fast MGB qRT-PCR Kit and GAPD (FAM-MGB) TaqMan® Endogenous Control primer/probe set (Applied Biosystems). Triplicate reactions for each RNA quantity were incubated for 5 min at 50ºC followed by 30s at 95ºC and then 40 cycles of 3s, 95ºC; 20s, 60ºC on a Mastercycler® ep realplex S (Eppendorf). Total run time was under 41 minutes.

More Performance Data

• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to TaqMan® One-Step RT-PCR Reagents (Applied Biosystems)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to QuantiFast™ Probe RT-PCR Kit (Qiagen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to iScript™ One-Step RT-PCR Kit for Probes (Bio-Rad)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to TaqMan® One-Step RT-PCR Reagents (Applied Biosystems)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to QuantiFast™ Probe RT-PCR Kit (Qiagen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)
• qScript™ One-Step Fast MGB qRT-PCR Kit Comparison to iScript™ One-Step RT-PCR Kit for Probes (Bio-Rad)
• One-Step qRT-PCR Performance Comparison with TaqMan® Gene Expression Assays (FAM-MGB Probes): Improved Sensitivity of qScript™ One-Step Fast MGB qRT-PCR Kit vs. TaqMan® One-Step RT-PCR Master Mix Reagents Kit (Applied Biosystems)
• One-Step qRT-PCR Performance Comparison with TaqMan® Gene Expression Assays (FAM-MGB Probes): Improved Sensitivity of qScript™ One-Step Fast MGB qRT-PCR Kit vs. QuantiFast™ Probe RT-PCR Kit (Qiagen)
• One-Step qRT-PCR Performance Comparison with TaqMan® Gene Expression Assays (FAM-MGB Probes): Improved Sensitivity of qScript™ One-Step Fast MGB qRT-PCR Kit vs. SuperScript™ III Platinum® One-Step qRT-PCR Kit (Invitrogen)

qScript™ One-Step qRT-PCR Kit: Performance Data

Broad Dynamic Range One-Step qRT-PCR (GAPD)
GAPD mRNA was amplified from log-fold serial dilutions of HeLa cell total RNA (1 ug to 1 pg) in 25-uL volumes with the qScript™ One-Step qRT-PCR Kit and GAPD TaqMan® Endogenous Control primer/probe set (Applied Biosystems) according to the manufacturers protocol. Averaged plots of quadruplicate qRT-PCR reactions are shown.

More Performance Data

• High Sensitivity One-Step qRT-PCR (DKK1)
• Duplexed One-Step qRT-PCR of ACTB and nptII RNAs
• High Efficiency Duplex One-Step qRT-PCR
• qScript™ One-Step qRT-PCR Kit Comparison to iScript™ One-Step RT-PCR for Probes (Bio-Rad)
• qScript™ One-Step qRT-PCR Kit Comparison to SuperScript™ III Platinum One-Step qRT-PCR Kit (Invitrogen)
• qScript™ One-Step qRT-PCR Kit Comparison to TaqMan® One-Step RT-PCR Reagents (Applied Biosystems)