PerfeCTa™ qPCR ToughMix™
Difficult samples making your PCR unreliable? Whip your gene expression experiments into shape with the toughest mix around.
PerfeCTa qPCR ToughMixes enable expression analysis and other quantitative applications from crude samples such as lysates, blood spots, plant tissues and clinical samples.
Features & Benefits
- Bullet-Proof qPCR - easily overcome tough PCR inhibitors
- Short Run Times—fast cycling protocols enable more experiments
- Optical Clarity Technology - high precision in reduced reaction volumes
- Repeatable Results - hot-start polymerase highly stable at ambient temperature
Description
PerfeCTa qPCR ToughMix is a ready-to-use 2X concentrated hotstart PCR mix containing additives which prevent inhibition of PCR or quenching of fluorescent signal by common PCR inhibitors. AccuFast™ DNA Polymerase is a low DNA hot-start preparation in which residual DNA is undetectable.
Overcome PCR Troublemakers
Many sample types are rich in PCR inhibitors, see table below. These PCR scoundrels operate by shutting down PCR amplification at threshold concentrations, or by quenching fluorescent signal. Expensive or timeconsuming purification steps are no longer required to eliminate inhibitors—PerfeCTa qPCR ToughMix neutralizes problem-causing inhibitors in crude lysates and other difficult samples.
| Inhibitor | Common Sources | Reagent Performance | |
|---|---|---|---|
| Competitor | AccuStart ToughMix | ||
| Polyphenols | Plant Extracts |  |
|
| Humic Acids | Soil, Plant Tissue | |
|
| Hematin | Dried Blood, Blood Spots | |
|
| Hemoglobin | Blood | |
|
| Polysaccharides | Feces, Plant Tissue | |
|
| Melanin | Hair, Skin | |
|
Performance Data
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