Unbiased target pre-amplification is often required when working with limiting amounts of starting material (e.g. laser capture microdissections, FACS isolated cells or precious clinical specimens) or when working with nanoscale reaction volumes associated with microfluidic qPCR.

PerfeCTa PreAmp is a stabilized 5X concentrated reagent that delivers unbiased pre-amplification of up to 100 target cDNA sequences from as little as 100 pg of total RNA input and is compatible with either probe-based or dye-based qPCR detection chemistries.

PerfeCTa PreAmp is optimized to work seamlessly with PerfeCTa qPCR reagents

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  • PerfeCTa PreAmp SuperMix 5X concentrated, ready-to-use reaction cocktail for unbiased, selected enrichment of target sequences from limiting amounts of starting material for downstream gene expression profiling or targeted re-sequencing.