Conventional PCR

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.

Quantabio PCR reagents are formulated with ultrapure AccuStart™ DNA polymerase, which contains a stringent antibody hotstart to ensures specific and efficient primer extension only after activation at 94°C.  Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Available in a variety of optimized formulations designed to support specific PCR applications and starting materials.

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Featured Products

  • AccuStart Taq DNA Polymerase HiFi Contains 3'-exonuclease (proof-reading) polymerase for robust amplification of large, complex DNA fragments up to 20 kb in length with reliable sequence fidelity
  • AccuStart II PCR SuperMix

    A stabilized, ready-to-use (2X) reaction cocktail for robust general purpose PCR amplification of DNA fragments up to 4 kb. GelTrack version streamlines gel electrophoresis analysis

  • AccuStart II Taq DNA Polymerase Ultrapure, high-yielding Taq DNA polymerase mutant with stringent antibody hot start.
  • AccuStart II PCR ToughMix A stabilized, ready-to-use (2X) reaction cocktail for sensitive PCR amplification of DNA templates using crude extracts, clinical specimens, plant tissues, animal tissues, environmental samples and complex food matrices
  • 5PRIME HotMaster Taq DNA Polymerase Innovative Hot-Start/Cold-Stop technology for highly specific Hot-Start PCR without enzyme activation
  • 5PRIME HotMasterMix Highly specific amplification with minimal handling
  • AccuStart II GelTrack PCR SuperMix AccuStart II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. Premixed with electrophoretic dye for gel electrophoresis.