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PCR & qPCR

Non-specific extension of primers at low temperature is a common cause of PCR artifacts and poor assay specificity and sensitivity.

Quantabio PCR reagents are formulated with ultrapure AccuStart™ DNA polymerase, which contains a stringent antibody hotstart to ensures specific and efficient primer extension only after activation at 94°C.  Our enzymes are rigorously purified to remove host E. coli genomic DNA and are ideally suited for applied testing applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy targets.

Rely on quality Quantabio manufactured reagents that maximize assay performance, minimize errors and deliver reliable, reproducible results.

Featured Products

  • PerfeCTa qPCR ToughMix A 2X concentrated ready-to-use reaction cocktail for quantitative DNA detection with crude extracts or inhibitory starting materials such as clinical or environmental specimens, plant tissues, animal tissues, and complex food matrices
  • PerfeCTa FastMix II An advanced 2X concentrated qPCR SuperMix reagent optimized to support both fast and standard thermal cycling conditions with probe-based real-time quantitative PCR assays
  • repliQa™ HiFi Assembly Mix The repliQa™ HiFi Assembly Mix simplifies the construction of recombinant DNA through scarless assembly of multiple DNA fragments in a single, isothermal reaction.
  • PerfeCTa Multiplex qPCR ToughMix

    An advanced 5X concentrated ToughMix reagent optimized to support highly-multiplexed real-time qPCR amplification across a range of inhibitory sample types and crude extraction methods

  • AccuStart II PCR ToughMix A stabilized, ready-to-use (2X) reaction cocktail for sensitive PCR amplification of DNA templates using crude extracts, clinical specimens, plant tissues, animal tissues, environmental samples and complex food matrices