A faster, smaller, better way to qPCR

Features & Benefits

Ultra-Fast Data Acquisition

35 cycles in 25 minutes

Unrivaled Performance

Detect 2-fold expression level differences

Portable & Compact

4.5 lbs - transport without ever calibrating

Scalable & Wireless
Connect up to 10 instruments
(48 samples/instrument)

Magnetic Induction Technology

Eliminate variability vs block-based cyclers

Q is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

Q uses a patented magnetic induction technology to rapidly heat samples coupled with fan forced air for cooling to acquire data in only 25 minutes. Available in 2 or 4 channel models, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.

Q’s miniature speaker-size and 4.5 pound weight make it the most portable and versatile qPCR cycler on the market without ever needing to calibrate. Q also provides scalability as each instrument can process up to 48 samples per run and up to 10 Q’s can be connected to a single computer wirelessly via bluetooth enabling up to 480 samples to be processed simultaneously.

A key difference is that Q incorporates a unique spinning aluminum rotor providing superior temperature uniformity of
± 0.05°C versus traditional block-based real time cyclers which rely on multiple peltier heating blocks that can create edge effects resulting in sample variation. Not only does the data give you superior reproducibility, repeatability but enables detection of 2-fold gene expression level differences as well as identification of difficult class IV SNP’s requiring melt temperature resolutions of 0.1°C.

Who wouldn’t want to take one for a spin?

Ultra-Fast Data Acquisition

Generate high quality data, fast!

Ultra-Fast Data Acquisition


Ultra-Fast Data Acquisition

5 point, 2x dilution series of Hepatitis B virus (HBV) cDNA template
Starting amount of 3E+06 copies (n = 4 each)
Efficiency = 90% (standard curve method); R² = 0.99
Time to complete run (including melt) = 26 min


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  • Q’s speed is the fastest in the industry
  • Don’t sacrifice on the performance quality of your qPCR
  • Completing runs in as little as 25 minutes* is the new standard

*25 minute cycle times obtained with fast cycling master mixes and short amplicon assay designs targeting cDNA


Unrivaled Performance

Detect 2-fold Differences

Unrivaled Performance


Confidently detect small differences

1.2
Manganese superoxide dismutase gene (MnSOD)
Eight point, 2x dilution series of human genomic DNA (n = 4 each)
Efficiency = 98% (standard curve method)
R² = 1.00

1.3
Five point dilution series of HBV plasmid cDNA template (n = 4 each)
5 picogram difference between standards
Efficiency = 98% (standard curve method)
R² = 0.99


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  • Confidently detect small differences
  • High thermal uniformity and reproducibility
  • Detect differences within a single cycle

Wide Linear Dynamic Range

Wide Linear Dynamic Range


Wide Linear Dynamic Range

10 point, 10x dilution series of Hepatitis B virus (HBV) cDNA template Starting amount of 3E+09 copies (n = 3 each) over 10 logs Efficiency = 95% (standard curve method) R² = 0.99


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  • Ultra-sensitive detection down to single digit copies of DNA
  • Q performs reliably across a wide dynamic range


Portable & Compact

A qPCR cycler the size of a speaker!

Q is the most portable and compact cyler on the market occupying ¼ the size of current qPCR platforms. The small footprint is enabled by the unique rotary and magnetic induction technology.

Portable & Compact


A qPCR cycler the size of a speaker!

Don’t be fooled by its small size. Big things are happening inside.

  • Fixed optics & no moving parts
  • Never needs optical alignment or calibration
  • No reference dyes or cross talk compensation required
  • Utilizes proprietary 0.1 ml 4-strip tubes & caps
  • supporting volumes of 5 – 30 μl
  • High speed centrifugation ensures sample spin down
  • Prevents evaporation & condensation with pre-loaded oil in tubes


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  • Fixed optics & no moving parts
  • Never needs optical alignment or calibration
  • No reference dyes or cross talk compensation required
  • Utilizes proprietary 0.1 ml 4-strip tubes & caps supporting volumes of 5 – 30 μl
  • High speed centrifugation ensures sample spin down
  • Prevents evaporation & condensation with pre-loaded oil in tubes


Scalable & Wireless

Flexibility from 48 to 480 samples

Each Q cycler can process up to 48 samples per run and up to 10 cyclers can be connected to a single computer wirelessly to provide the desired scalability.

Scalable & Wireless


Flexibility from 48 to 480 samples

Each Q cycler can process up to 48 samples per run and up to 10 cyclers can be connected to a single computer wirelessly to provide the desired scalability.
Q’s advanced data analysis software makes combining a single data set from multiple runs from multiple cylers seamlessly simple.
High reproducibility from thermal and optical performance ensures data from different runs and cyclers look like it was generated on the same instrument and the same run.
Lastly, anyone can setup and run the Q as it is plug-and play right of the box.


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  • Combine single data sets from multiple runs from multiple cyclers
  • Data from different runs and cyclers look a like
  • Q is plug-and-play

Warranty Policy

Click Here for the Warranty Policy for Q qPCR Instruments

Performance Data

Ultra-Fast Data Acquisition


Generate high quality data, fast!

5 point, 2x dilution series of Hepatitis B virus (HBV) cDNA template
Starting amount of 3E+06 copies (n = 4 each)
Efficiency = 90% (standard curve method); R² = 0.99
Time to complete run (including melt) = 26 min

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Unrivaled Performanc


Detect 2-fold Differences

Manganese superoxide dismutase gene (MnSOD)
Eight point, 2x dilution series of human genomic DNA (n = 4 each)
Efficiency = 98% (standard curve method)
R² = 1.00

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Wide Linear Dynamic Range


Q provides ultra-sensitive detection down to single digit copies of DNA.

10 point, 10x dilution series of Hepatitis B virus (HBV) cDNA template
Starting amount of 3E+09 copies (n = 3 each) over 10 logs
Efficiency = 95% (standard curve method)
R² = 0.99

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Portable & Compact


A qPCR cycler the size of a speaker!

Don’t be fooled by it’s small size. Big things are happening inside.

  • Fixed optics & no moving parts
  • Never needs optical alignment or calibration
  • No reference dyes or cross talk compensation required
  • Utilizes proprietary 0.1 ml 4-strip tubes & caps
  • supporting volumes of 5 – 30 μl
  • High speed centrifugation ensures sample spin down
  • Prevents evaporation & condensation with pre-loaded oil in tubes


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>

Scalable & Wireless


Flexibility from 48 to 480 samples

Each Q cycler can process up to 48 samples per run and up to 10 cyclers can be connected to a single computer wirelessly to provide the desired scalability.
Q’s advanced data analysis software makes combining a single data set from multiple runs from multiple cylers seamlessly simple.
High reproducibility from thermal and optical performance ensures data from different runs and cyclers look like it was generated on the same instrument and the same run.
Lastly, anyone can setup and run the Q as it is plug-andplay right of the box.

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Request a Demo

Register / Download Software


Press Release
Quantabio Launches Compact qPCR Cycler and Redefines Industry’s Pricing and Performance Standards for Molecular Testing Applications"

Read the press release


What Customers Say
We had the pleasure of testing out the ToughMix Mastermix and the “Q” RT-PCR machine in Mbabane Swaziland in our Tuberculosis Centre of Excellence.” As... Read More We had the pleasure of testing out the ToughMix Mastermix and the “Q” RT-PCR machine in Mbabane Swaziland in our Tuberculosis Centre of Excellence.” As it’s name implies, the ToughMix stood up to its name and made the 38 hour trip from Houston to Mbabane, Swaziland and had beautiful Ct curves. Similarly, the Q PCR machine was an ease to travel with as carry on luggage. It’s 25 minute run gave Ct curves identical to our existing, and much bulkier, RT-PCR machine that was already on site in Swaziland. Considering it’s size, intuitive software, and near perfect reproducible Ct curves, we’ve decided to add the Q as our workhorse RT-PCR machine"
Assistant Professor
Infectious Diseases Global and Immigrant Health Baylor College of Medicine

Details

  • Contents

    Specifications

    Front Back

    Physical

    Height 5.1 in
    Width 5.9 in
    Length 5.9 in
    Weight 4.5 lbs

    Mic qPCR Cycler Specifications Front View     Mic qPCR Cycler Specifications Top View 


    Thermal Performance

    Temperature Accuracy ±0.25°C
    Temperature Uniformity ±0.05°C
    Ramp Rates Heating 4°C/s
    Cooling 3°C/s
    Temperature Input Range 40 – 99°C


    Optical

    Detectors Photodiode per channel
    Excitation Sources High power LED for each channel
    Channels Green Ex 465nm Em 510nm
    Yellow Ex 540nm Em 570nm
    Orange Ex 585nm Em 618nm
    Red Ex 635nm Em 675nm
    Acquisition Time 1 second


    Reaction Tubes

    Samples per Instrument 48
    Reaction Volume Range 5 – 30µL


    Operating Environment

    Temperature 18 – 35ºC
    Relative Humidity 20 – 80%

  • Related Resources
    Product Flyers
    Product Manuals
    Technical Notes
    FAQs
    How can I find help about the Q-qPCR?
    The best initial resource is the Q-qPCR manual. You can access the manual by clicking on the ‘?’ icon located at the top left hand side of the software, and selecting the Q-qPCR manual option. Once open, you can scroll to the relevant section or use the ctrl+F search function to find what you need.
    Which fluorophores can I use with the Q?
    The Q-qPCR’s optical detectors will work optimally with FAM (green), Cal Fluor Orange 560 (yellow detector), Cal Fluor Red 610 (orange channel) and Quasar 670 (red channel). This combination of fluorophores will produce less than 3% crosstalk between any/all channel/s used. Other fluorophores with similar spectral properties will also be suitable. For example, Texas Red/ROX have very similar spectral properties to Cal Fluor Red 610, and can be measure on the orange channel. A list of popular fluorophores that are suitable for a particular detector are available while setting up the assay chemistry, and a more comprehensive chart is provided in the Q-qPCR manual (page 143).
    Can I use the Q-qPCR for fieldwork?
    The Q-qPCR is well suited for fieldwork. You can run the machine from any power source, using a power inverter as required. Because you don’t need to calibrate for optics or temperature, and because of its small size/weight, the Q is ideal to transport to field sites while retaining functionality – it stays plug-and-play when other machines would need calibration. Also see our Technical Note: Q in the Field - Alternative Power Sources
    Can the samples be used for post-PCR analysis when the tubes have the oil overlay?
    Yes, you can break through the oil layer with a pipette tip to remove your PCR reaction without the oil.
    How fast can the Q complete a run?
    While this will vary according to your particular assay (different annealing temperatures and hold times), the Q can typically perform a 35 cycle qPCR run complete with melt curve in under 25 min.
    Click here to see all FAQs
  • Testimonials

    We had the pleasure of testing out the ToughMix Mastermix and the “Q” RT-PCR machine in Mbabane Swaziland in our Tuberculosis Centre of Excellence.” As it’s name implies, the ToughMix stood up to its name and made the 38 hour trip from Houston to Mbabane, Swaziland and had beautiful Ct curves. Similarly, the Q PCR machine was an ease to travel with as carry on luggage. It’s 25 minute run gave Ct curves identical to our existing, and much bulkier, RT-PCR machine that was already on site in Swaziland. Considering it’s size, intuitive software, and near perfect reproducible Ct curves, we’ve decided to add the Q as our workhorse RT-PCR machine.

    Assistant Professor

    Infectious Diseases Global and
    Immigrant Health Baylor College of Medicine

Ordering Information

Related Products

Q qPCR Instrument Registration and Software Download


Please select if you want to register your Q qPCR instrument or download the Q software for evaluation or demo:

Please select:

  • Q qPCR Instrument Registration
  • Q qPCR Instrument Demo Software Download

Please register your new Q instrument to initiate the 2-year warranty.

By completing the form below, you will also receive:

  • Free software updates for the life of the instrument
  • Access to qPCR reagents promotions and offers
  • Announcements of new products and applications

Please enter your contact information in the fields below to download the Q demo software

  • q qPCR Instrument Information
  • Contact Information
  • I give Quantabio or an authorized Quantabio distributor permission to contact me for product updates and news.
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