Cat# 95067-100	100 x 50-µl rxns (2 x 1.25 ml)	$181.00
Cat# 95067-500	500 x 50-µl rxns (10 x 1.25 ml)	$817.00
Cat# 95067-02K	2000 x 50-µl rxns (1 x 50 ml)	$3042.00

Features & Benefits

Buffer optimization for SYBR® Green stability, ensuring reliable performance
High sensitivity across a broad range of template concentrations
Highly specific amplification over a broad dynamic range
Superior reproducibility and precision

Description

PerfeCTa® SYBR® Green SuperMix, UNG, for iQ™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR on Bio-Rad iCycler iQ™ systems. The proprietary buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies to Taq that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Additionally, the dNTP mix in this SuperMix contains dUTP in place of dTTP. Inclusion of uracil-N-glycosylase (UNG) prevents amplification of carry-over contamination from previous dU-containing PCRs.

Real-Time PCR Instrument Compatibility

BioRad iCycler iQ™
BioRad MyiQ™
BioRad iQ™5

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dUTP), AccuStart Taq DNA Polymerase, UNG, SYBR Green I dye, 20 nM fluorescein, and stabilizers.

Storage conditions

PerfeCTa® SYBR Green SuperMix, UNG, for iQ™ is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.