AccuStart™ Taq DNA Polymerase
| Cat# 95061-250 | 250 units; 5 units/µl | $162.00 |  |
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| Cat# 95061-01K | 1000 units; 5 units/µl | $595.00 | |
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| Cat# 95061-05K | 5000 units; 5 units/µl | $2704.00 | |
Description
AccuStart Taq DNA Polymerase is a recombinant Taq DNA polymerase preparation which contains monoclonal antibodies that bind to the polymerase and keep it inactive before PCR thermal cycling. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active Taq DNA polymerase. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. Activated AccuStart Taq DNA polymerase possesses 5'→3' DNA polymerase activity and a double-strand specific 5'→3' exonuclease. The polymerase does not have 3'-exonuclease activity and is free of any contaminating endo or exonuclease activities. One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Contents
- AccuStart Taq DNA polymerase - 5 units/µL in 50% glycerol, 20 mM Tris-HCl, 40 mM NaCl, 0.1 mM EDTA, and stabilizers.
- 10X PCR Buffer - 0.2M Tris-HCl (pH 8.4), 0.5 M KCl
- 50 mM magnesium chloride - 50 mM MgCl2
Storage conditions
AccuStart Taq DNA polymerase is stable for 2 years when stored in a constant temperature freezer at -20°C.
