AccuStart™ PCR SuperMix
| Cat# 95060-100 | 100 x 50-µl rxns (2 x 1.25 ml) | $135.00 |  |
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| Cat# 95060-500 | 500 x 50-µl rxns (10 x 1.25 ml) | $595.00 | |
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| Cat# 95060-02K | 2000 x 50-µl rxns (1 x 50 ml) | $2055.00 | |
Features & Benefits
- High yield, high sensitivity
- Precise amplification - hot-start technology ensures specific and efficient primer extension
- Superior reproducibility and precision
- Convenient reaction assembly at room temperature
Description
AccuStart PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb. It contains all components, except primers and template. AccuStart PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. A key component is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.
Contents
- 2X reaction buffer containing 3 mM MgCl2, 0.4 mM each dNTP (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, and stabilizers.
Storage conditions
AccuStart PCR SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C. For convenience, it may be stored unfrozen at +2 to +8°C for up to 12 months. Repeated freezing and thawing does not impair product performance.
