Monday, September 6, 2010    

Home -> qPCR - SYBR® Green I Detection -> Cat# 95055


PerfeCTa® SYBR® Green SuperMix, ROX™

Cat# 95055-100 100 x 50-µl rxns (2 x 1.25 ml) $172.00   Order
Cat# 95055-500 500 x 50-µl rxns (10 x 1.25 ml) $778.00   Order
Cat# 95055-02K 2000 x 50-µl rxns (1 x 50 ml) $2897.00   Order

Manual MSDS Free Sample

Features & Benefits

  • Buffer optimization for SYBR® Green stability, ensuring reliable performance
  • High yield, high sensitivity
  • Highly specific amplification
  • Improved reproducibility and assay precision

Description

PerfeCTa® SYBR® Green SuperMix, ROX™ is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR on Applied Biosystems 7000, 7300, 7700, or 7900 instruments. The proprietary buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. This supermix provides the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology because this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Real-Time PCR Instrument Compatibility

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Contents

  • 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, SYBR Green I dye, ROX Reference Dye, and stabilizers.

Storage conditions

PerfeCTa® SYBR Green SuperMix, ROX is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.