Cat# 95054-100	100 x 50-µl rxns (2 x 1.25 ml)	$172.00
Cat# 95054-500	500 x 50-µl rxns (10 x 1.25 ml)	$778.00
Cat# 95054-02K	2000 x 50-µl rxns (1 x 50 ml)	$2897.00

Features & Benefits

Buffer optimized for SYBR® Green stability, ensures reliable performance
High yield, high sensitivity
Highly specific amplification
Superior reproducibility and precision

Description

PerfeCTa® SYBR® Green SuperMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template for real-time quantitative PCR systems that do not require an internal reference dye. The proprietary buffer and stabilizers have been optimized exclusively for SYBR® Green I qPCR to deliver maximum PCR efficiency, sensitivity, and robust fluorescent signal. This supermix provides the highest level of specificity to reduce the occurrence or delay the detection of primer-dimer and other non-specific artifacts. Highly specific amplification is crucial to successful qPCR with SYBR® Green I technology since this dye binds to and detects any dsDNA generated during amplification. A key component of this supermix is AccuStart™ Taq DNA polymerase which contains monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (2 minutes at 95°C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Real-Time PCR Instrument Compatibility

Roche LightCycler™ 480
Qiagen/Corbett Rotor-Gene® Q
Qiagen/Corbett Rotor-Gene® 6000
Qiagen/Corbett Rotor-Gene® 3000
Eppendorf Mastercycler® ep realplex
Eppendorf Mastercycler® ep realplex2 s
Cepheid SmartCycler®
Bio-Rad CFX96™
Bio-Rad CFX384™
Bio-Rad/MJ Opticon®
Bio-Rad/MJ Opticon 2
Bio-Rad/MJ Chromo4™
Bio-Rad MiniOpticon™

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), AccuStart Taq DNA Polymerase, SYBR Green I dye, and stabilizers.

Storage conditions

PerfeCTa® SYBR Green SuperMix is stable for 1 year when stored in a constant temperature freezer at -20°C, protected from light. For convenience, it may be stored unfrozen at +2 to +8°C for up to 6 months. Repeated freezing and thawing of the supermix is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20°C.