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AccuStart™ Taq DNA Polymerase and AccuStart™ PCR SuperMix provide high specificity amplification for all PCR applications. An antibody mediated hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly.
- High yield, high sensitivity - sensitively amplify up to 4 kb single-copy genes
- Precise amplification - antibody-based hot-start technology
- Superior reproducibility - ready-to-use SuperMix enhances reproducibility and precision
AccuStart™ PCR SuperMix: Performance Data
Amplification of 4-kb fragment of the beta-globin gene from human genomic DNA
AccuStart™ PCR SuperMix was used to amplify a 4-kb fragment from the beta-globin gene from varying amounts of human genomic DNA as indicated in the figure. PCR amplification was for 95°C, 1 min followed by 35 cycles of 95 °C, 20s; 58 °C, 20s; 68 °C, 5 min. 1/10th of each PCR was analyzed on a 0.8% agarose, 0.5X TBE gel with ethidium bromide staining.
Reactions were incubated at 37 °C for 30 min prior to PCR to demonstrate efficacy and stringency of the antibody-mediated AccuStart Hot-Start. Control reactions without hot-start failed to amplify the specific target fragment (data not shown). AccuStart qPCR SuperMix provides rapid and complete recovery of fully active, unmodified Taq polymerase activity. This is crucial for amplification of long fragments from complex templates and robust results for routine PCR applications.
10mM dNTP Mix: Performance Data
Effect of dNTP Quality on qPCR: Low Copy Precision
IL1-b was assayed by TaqMan® qPCR in 12 replicate reactions containing 25 pg of human genomic DNA (approximately 15 copies) using either Quanta's qPCR-Qualified dNTP Mix or PCR-Grade dNTP mix from a leading supplier. Delayed Cts with higher standard deviation and higher frequency of failed qPCRs was evident in dNTP preparations that did not meet Quanta's rigorous qPCR standards.
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