Signal-to-noise ratio

Obtain higher and discrete fluorescent signals for more accurate allele calls. Comparison of TaqMan® Genotyping Master Mix and PerfeCTa™ qPCR SuperMix for allelic discrimination of an A/G transition in CYP2C19. Genotyping reactions were run on a panel of 94 gDNA samples (3 ng) and two NTCs according to the each manufacture’s recommend conditions with TaqMan® SNP Assay C__25986767_70 (Applied Biosystems).