Speed

Obtain equivalent SNP results with ½ primer-probe in ½ the time. Genotyping reactions were performed on a panel of 94 gDNA samples (3 ng) and 2 NTCs with either TaqMan® Genotyping Master Mix and 1X TaqMan® SNP Assay C__12050942_10 or PerfeCTa™ qPCR SuperMix with 1/2X primer/probe mix. Cycling protocols were as described in Table 1 followed by allelic discrimination analysis on the 7500 System (Applied Biosystems).