qPCR SYBR Green I Detection
Select Your Real Time Instrument
Gene Expression Analysis
PerfeCTa SYBR Green FastMix products are the optimal choice for routine quantitative PCR applications such as transcript profiling, chromatin immune-precipitation (ChIP), and copy-number assays.
FastMixes are formulated for optimal PCR efficiency so relative and absolute quantification results can be accurate.
You can choose from fast or standard cycling conditions, and two or three stage protocols.
Optical Clarity Technology ensures reproducibility and formulations are available for all real-time platforms.
Hot-start technology maximizes specificity while enabling bench-top reaction assembly.
microRNA Profiling
PerfeCTa SYBR Green SuperMix products are recommended for microRNA quantification and other applications where PCR specificity is a challenge.
In the trade-off between PCR efficiency and specificity the SYBR Green SuperMix provides a more stringent option enabling specific amplification of short sequences such as small RNAs.
Standard cycling times are recommended for amplicons up to 250 bases. Optical Clarity Technology ensures reproducibility and formulations are available for all real-time platforms.
Hot-start technology maximizes specificity while enabling bench-top reaction assembly.
Related Tools
| ACTB PerfeCTa Reference Gene Assay Human ACTB (Beta-actin) Cat# 95121-250 500 x 20-µl rxns $65.00 |
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| B2M PerfeCTa Reference Gene Assay Human B2M (Beta-2-microglobulin) Cat# 95122-250 500 x 20-µl rxns $65.00 |
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| GAPDH PerfeCTa Reference Gene Assay Human GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) Cat# 95123-250 500 x 20-µl rxns $65.00 |
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| GUSB PerfeCTa Reference Gene Assay Human GUSB (Beta-glucuronidase) Cat# 95124-250 500 x 20-µl rxns $65.00 |
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| HPRT1 PerfeCTa Reference Gene Assay Human HPRT1 (Hypoxanthine-guanine phosphoribosyltransferase) Cat# 95125-250 500 x 20-µl rxns $65.00 |
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| PPIA PerfeCTa Reference Gene Assay Human PPIA (Peptidylprolyl isomerase A (cyclophilin A)) Cat# 95126-250 500 x 20-µl rxns $65.00 |
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| TFRC PerfeCTa Reference Gene Assay Human TFRC (Transferrin receptor) Cat# 95127-250 500 x 20-µl rxns $65.00 |
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| UBC PerfeCTa Reference Gene Assay Human UBC (Ubiquitin C) Cat# 95128-250 500 x 20-µl rxns $65.00 |
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| YWHAZ PerfeCTa Reference Gene Assay Human YWHAZ (Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide) Cat# 95129-250 500 x 20-µl rxns $65.00 |
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| RPL13A PerfeCTa Reference Gene Assay Human RPL13A (60S ribosomal protein L13a) Cat# 95130-250 500 x 20-µl rxns $65.00 |
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Click here for information on legacy Bio-Rad formulations.




