Tuesday, February 7, 2012    

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qPCR SYBR Green I Detection

Select Your Real Time Instrument

Gene Expression Analysis

PerfeCTa SYBR Green FastMix products are the optimal choice for routine quantitative PCR applications such as transcript profiling, chromatin immune-precipitation (ChIP), and copy-number assays.
FastMixes are formulated for optimal PCR efficiency so relative and absolute quantification results can be accurate.
You can choose from fast or standard cycling conditions, and two or three stage protocols.
Optical Clarity Technology ensures reproducibility and formulations are available for all real-time platforms.
Hot-start technology maximizes specificity while enabling bench-top reaction assembly.

microRNA Profiling

PerfeCTa SYBR Green SuperMix products are recommended for microRNA quantification and other applications where PCR specificity is a challenge.
In the trade-off between PCR efficiency and specificity the SYBR Green SuperMix provides a more stringent option enabling specific amplification of short sequences such as small RNAs.
Standard cycling times are recommended for amplicons up to 250 bases. Optical Clarity Technology ensures reproducibility and formulations are available for all real-time platforms.
Hot-start technology maximizes specificity while enabling bench-top reaction assembly.

Related Tools

PerfeCTa Reference Gene Assays
ACTB PerfeCTa Reference Gene Assay
Human ACTB (Beta-actin)
Cat# 95121-250        500 x 20-µl rxns        $65.00
OrderManual
B2M PerfeCTa Reference Gene Assay
Human B2M (Beta-2-microglobulin)
Cat# 95122-250        500 x 20-µl rxns        $65.00
OrderManual
GAPDH PerfeCTa Reference Gene Assay
Human GAPDH (Glyceraldehyde 3-phosphate dehydrogenase)
Cat# 95123-250        500 x 20-µl rxns        $65.00
OrderManual
GUSB PerfeCTa Reference Gene Assay
Human GUSB (Beta-glucuronidase)
Cat# 95124-250        500 x 20-µl rxns        $65.00
OrderManual
HPRT1 PerfeCTa Reference Gene Assay
Human HPRT1 (Hypoxanthine-guanine phosphoribosyltransferase)
Cat# 95125-250        500 x 20-µl rxns        $65.00
OrderManual
PPIA PerfeCTa Reference Gene Assay
Human PPIA (Peptidylprolyl isomerase A (cyclophilin A))
Cat# 95126-250        500 x 20-µl rxns        $65.00
OrderManual
TFRC PerfeCTa Reference Gene Assay
Human TFRC (Transferrin receptor)
Cat# 95127-250        500 x 20-µl rxns        $65.00
OrderManual
UBC PerfeCTa Reference Gene Assay
Human UBC (Ubiquitin C)
Cat# 95128-250        500 x 20-µl rxns        $65.00
OrderManual
YWHAZ PerfeCTa Reference Gene Assay
Human YWHAZ (Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide)
Cat# 95129-250        500 x 20-µl rxns        $65.00
OrderManual
RPL13A PerfeCTa Reference Gene Assay
Human RPL13A (60S ribosomal protein L13a)
Cat# 95130-250        500 x 20-µl rxns        $65.00
OrderManual

Click here for information on legacy Bio-Rad formulations.