AccuStart™ Genotyping ToughMix™
Difficult samples causing unreliable genotyping results?
Whip them into shape with the toughest PCR mix around.
AccuStart Genotyping ToughMixes enable genetic analysis including TaqMan® genotyping directly from crude samples such as lysates, blood spots, plant tissues and clinical samples.
Features & Benefits
- Bullet-Proof Genotyping - easily overcome tough PCR inhibitors
- Short Run Times—fast cycling protocols enable more experiments
- Signal Optimization - robust genotyping with low primer & probe concentration
- Flexible Workflow - assembled reactions stable 3 days pre/post-PCR
Description
AccuStart Genotyping ToughMix is a ready-to-use 2x concentrated hot-start PCR mix containing additives which prevent inhibition of PCR or quenching of fluorescent signal by common PCR inhibitors. AccuStart DNA Polymerase is a low DNA hot-start preparation in which residual DNA is undetectable.
Overcome PCR Troublemakers
Many sample types are rich in PCR inhibitors, see table below. These scoundrels operate by shutting down PCR amplification at threshold concentrations, or by quenching fluorescent signal. Expensive or time-consuming purification steps are no longer required to eliminate inhibitors - AccuStart Genotyping ToughMix neutralizes problem causing inhibitors in crude lysates and other difficult samples.
| Inhibitor | Common Sources | Reagent Performance | |
|---|---|---|---|
| Competitor | AccuStart ToughMix | ||
| Polyphenols | Plant Extracts |  |
|
| Humic Acids | Soil, Plant Tissue | |
|
| Hematin | Dried Blood, Blood Spots | |
|
| Hemoglobin | Blood | |
|
| Polysaccharides | Feces, Plant Tissue | |
|
| Melanin | Hair, Skin | |
|
Performance Data
Click thumbs to enlarge
Fig.1 ToughMix vs Competitor
Fig.2 Influence of PCR inhibitor
