TaqMan® Genotyping
PerfeCTa® qPCR Supermixes for more robust, convenient and
economic genotyping
PerfeCTa® qPCR Supermixes raise the bar for performance and economy by generating accurate allele calls while requiring less probe and primer. Greater productivity is achieved through faster run times, ease of use and stability.
Features & Benefits
- Shorter Run Times – enabling more experiments per day
- Robust Signal – use ½ the primer and probe concentration
- Convenience & Reliability – stability for 3 days pre-PCR and 5 days post-PCR
- Simplified & Robust Allele Calling – fewer sample failures or re-work
Description
PerfeCTa® qPCR SuperMixes are 2X concentrated, ready-to-use reaction cocktails containing all components except primers, probe and template for any real-time quantitative PCR instrument. AccuStart™ Taq enables faster activation and extension rate resulting in enhanced productivity through faster cycling (see table comparison). Superior signal generation of PerfeCTa® SuperMixes allows greater economy through use of lower primer and probe concentrations compared to other leading products (see Fig. 1). Additionally PerfeCTa® SuperMixes yield reliable discrimination of SNPs in difficult targets (see Fig. 2).
Comparison Conventional vs. PerfeCTa®
| TaqMan® GenotypingMasterMix | PerfeCTa™ qPCR SuperMix | |
| Activation | 10 min, 95°C | 5 min, 95°C |
| PCR Cycling | 15s, 95°C / 60s, 60°C | 5s, 95°C / 30s, 60°C |
| Cycle Time (40 cycles) | 60 min (excl. ramp time) | 29 min (excl. ramp time) |
Quanta SuperMixes include optimized buffers and stabilizers for TaqMan® probe and MGB-probe chemistries. The enhanced specificity of PerfeCTa® SuperMixes suppress cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. This provides discrete and well-separated clusters for clear genotyping results. Tight control of AccuStart™ Taq polymerase activity conveys exceptional pre-PCR stability for convenient room temperature reaction assembly and flexible scheduling of PCR runs (see Fig. 3). Stable fluorescent signal allows automated scanning of results without compromising assay performance (see Fig. 4).
Product List Genotyping
Performance Data
Click thumbs to enlarge
Fig.1 Speed
Fig.2 Signal-to-noise ratio
Fig.3 Pre-PCR Stability
Fig.4 Post-PCR Stability
