AccuMelt™ HRM High Resolution Melt Analysis SuperMix
Accurately characterize genetic variations from novel mutations to rare polymorphisms and more.
AccuMelt HRM SuperMix provides discrete differences in melt temperature and curve shape to allow discrimination of DNA sequence differences amongst different samples. AccuMelt is suitable for a broad range of applications including SNP analysis, mutation scanning, species identification and DNA methylation analysis.
Features and Benefits
- See subtle differences in sequence clearly – robust amplification ensures sufficient yield of products to generate discrete melt curves
- Accurate genotype calling – comparable or better performance than TaqMan® Genotyping
- Work with rare or precious samples – large range of template inputs possible
- Specificity – works with lower Mg2+ concentration than other systems thus enhancing assay accuracy
Description
AccuMelt HRM SuperMix is a ready-to-use 2X concentrated hot-start PCR mix containing SYTO® 9 fluorescent DNA-binding dye. AccuStart™ DNA Polymerase allows for room-temperature reaction assembly and storage at +4°C for 6 months.
Superior Resolution of Genotypes
SNP Genotyping is a useful application for HRM and illustrates the capabilities of AccuMelt HRM SuperMix. Genotypes are readily identified based on unique melting profiles depending on a sample’s sequence (Figure 1). Furthermore, AccuMelt HRM SuperMix gives superior resolution of difficult genotypes when compared to the leading competitor’s mix based on greater Tm differences observed for A -> T transversions (Figure 2).
Comparison to TaqMan genotyping
TaqMan Genotyping has been used successfully in SNP analysis and other allelic discrimination applications. This widely adopted standard in genotyping was used as a benchmark to assess the utility of HRM with our SuperMix. AccuMelt HRM was determined to be just as effective as TaqMan Genotyping in SNP analysis and was even able to call the genotype for a difficult sample which the TaqMan assay could not resolve (Figure 3).
Robust Amplification
Consistent robust amplification is critical to accuracy in HRM analysis. AccuMelt HRM SuperMix will drive all PCR amplifications to plateau regardless of the quantity of template input (Figure 4). This ensures accurate results regardless of the quantity of DNA available.
Order Information
| Cat# 95103-250 | 250 x 20µl rxns (2 x 1,25 ml) | $195.00 |  |
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| Cat# 95103-012 | 1250 x 20µl rxns (10 x 1,25 ml) | $881.00 | |
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| Cat# 95103-05K | 5000 x 20µl rxns (2 x 25 ml) | $3283.00 | |
Performance Data
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Fig.1 Single variant base model
Fig.2 Comparison AccuMelt vs Competitor
Fig.3 Accuracy of AccuMelt HRM Genotyping
Fig.4 High yield, high efficiency PCR
